摘要
目的探讨人肝细胞生长因子（hHGF）基因转染成骨细胞移植促进股骨头的早期血管新生，增强坏死股骨头的局部骨修复能力。方法36只新西兰兔随机分成对照组、移植转染hGHF基因组和未转染成骨细胞组。体外分离、培养胎兔颅骨中的成骨细胞，利用脂质体介导hHGF基因体外转染成骨细胞，同时建立兔激素性股骨头缺血坏死模型，然后将细胞通过髓芯减压后移植于坏死的股骨头内，组织形态学分析血管发生和骨修复情况。结果胎兔颅骨中分离培养的成骨细胞表达Ⅰ型胶原和碱性磷酸酶，hHGF基因转染成骨细胞体外及体内检测均有hHGF蛋白的表达。转染hHGF基因的成骨细胞组和成骨细胞组移植坏死的股骨头内的新生骨小梁与单纯髓芯减压组比较有差异性，同时基因转染组的新生血管数较对照组差异有统计学意义（P〈0．01）。结论hHGF基因转染可促进缺血股骨头的血管新生，并增加骨形成，显著促进坏死骨修复。
Objective To investigate the feasibility of transplanted osteoblasts transfected with hHGF gene to avascular necrosis of femoral head with increased neovascularization and to improve restore function. Methods The fetal rabbit osteoblasts were isolated and cultured successfully in vitro. Plasmid pcDNA3.1^（＋）- hHGF containing hHGF gene was transfected into the osteoblasts. Thirty-six ANFH model New Zealand adult rabbits were randomly divided into 3 groups, transplanted with osteoblasts transfected with pcDNA3.1^（＋）-hHGF plasmid, osteoblasts not transfected with pcDNA3.1^（＋）-hHGF plasmid, and injected with PBS medium.Angio genesis and repair of the femoral head were observed by histological and histomorphometric analysis. Results The fetal rabbit osteoblasts were isolated and cultured successfully in vitro. Osteoblasts were identified by alkaline phosphorase and type Ⅰ collagenase immunohistochemical staining with high purity. Stable expression of hHGF in pcDNA3.1^（＋）-hHGF transfected osteoblasts was confirmed. Osteoblasts transfected and not transfected with pcDNA3.1 ^（＋）-hHGF plasmid have more bone formation than only channel was drilled at 8 weeks; and osteoblasts transfected with pcDNA3.1 ^（＋）-hHGF plasmid have HGF protein expression, en- hanced neovasculariation, pathological examination showed that the bone and neovasculariation formation function in osteoblasts transfected with pcDNA3.1 ^（＋）-hHGF plasmid were significantly better than any of the other groups. Conclusion Transfection of hHGF gene enhances local angiogenesis in early time and improves bone formation. Combination of gene transfer, cell transplantation and core decompression surgery can improve restore function of the avascular necrosis of femoral head.
出处
《中华显微外科杂志》
CSCD
北大核心
2008年第1期35-38,83,共5页
Chinese Journal of Microsurgery
关键词
肝细胞生长因子
成骨细胞
基因转染
细胞移植
髓芯减压术
股骨头缺血性坏死
Hepatocyte growth factor
Osteoblast
Gene transfer
Cell transplantation
Core decompression
Avascular necrosis of femoral head
