摘要
目的:采用高效液相色谱法建立麻黄药材的指纹图谱,为其品质控制提供依据。方法:采用RP-HPLC,CLC phenyl(5μm4.6 mm×150 mm)色谱柱,乙腈-0.1%磷酸水溶液梯度洗脱,流速1.0 mL/min,检测波长215 nm。结果:10批产各地麻黄药材指纹图谱有17个共有峰,峰面积之和大于总峰面积的90%。对各产地的药材进行了相似度比较,建立了麻黄药材的HPLC指纹图谱。结论:采用该法可为控制麻黄药材的内在质量提供依据。
AIM：To study the HPLC fingerprints and to establish a sensitive and specific method for controlling the quality of Herba Ephedrae. METHODS ： HPLC method was set up ,using CLCphenyl column（5 μm 4, 6 mm × 150 mm） with gradient elution, the mobile phase was comprised of acetonitrile and phosphoric acid（0.1% ）, the UV detection wavelength was at 215 nm, with a flow rate of 1, 0 mL/min. RESULTS： There were 17 common peaks in the fingerprints of Herba Ephedrae based on 10 habitats. Sum of peak areas was larger than 90% total area. The similarity was compared and the HPLC fingerprints was set up. CONCLUSION： The quality of Herba Ephedrae can be controlled effectively by HPLC fingerprints.
出处
《中成药》
CAS
CSCD
北大核心
2008年第2期163-166,共4页
Chinese Traditional Patent Medicine
基金
新疆大学青年教师科研启动基金项目(QN040125)
