摘要
目的观察人参皂甙Rb1(Ginsenoside Rb1,GRb1)对大鼠脑缺血再灌注时神经细胞凋亡及Bcl-2和Bax表达的影响,探讨GRbl的神经保护作用机制。方法阻塞大鼠大脑中动脉2 h制备脑缺血再灌注模型,大鼠随机分为缺血再灌注组(I/R组)和GRbl给药组(GRb1组),GRb1组大鼠在再灌注后立即腹腔注射GRb1(40 mg/kg)。两组再按不同的再灌注时间(3 h、12 h、1 d、2 d、3 d、5 d和10 d,每时间点4只)分为7个亚组。分别用HE染色、TUNEL染色和免疫组化染色观察神经组织病理改变、细胞凋亡、Bcl-2和Bax的表达。结果与I/R组相比,GRb1能减轻缺血侧脑组织的病理改变,减少神经细胞凋亡数(12 h、1 d、2 d和3 d时P<0.05),上调Bcl-2阳性细胞数(12 h、1 d、3d、5 d和10 d时P<0.05)和降低Bax阳性细胞数(3 h、12 h、1 d、2 d、3 d、5 d和10 d时P<0.05)。结论GRbl对脑缺血再灌注损伤有保护作用,其机制与抑制神经元凋亡、促进Bcl-2表达和抑制Bax表达有关。
Objective To observe the effects of Ginsenoside Rb1（GRb1） on neuronal cell apoptosis and the expressions of Bcl-2 and Bax in rats after cerebral ischemia-reperfusion so as to investigate the neuroprotective mechanism of GRb1. Methdos The model of cerebral ischemia-reperfusion was established by occluding rat middle cerebral artery for 2 h. The rats were randomly divided into two groups： ischemia-reperfusion group（I/R group） and GRb1 treat group（GRb1 group）. GRb1 （40 mg/kg, i. p. ） was administered immediately to rats after the onset of reperfusion. Two groups were further subdivided 7 subgroups according to various reperfusion time （3 h,12 h,1 d,2 d,3 d,5 d and 10 d, n=4 per time point）. HE staining was used to observe histological features. TUNEL and immunohistochemical method were used to analyze the cell apoptosis and expressions of Bcl-2 and Bax, respectively. Results Compared with I/R group, GRb1 reduced pathological changes, and decreased the number of apoptotic neural cells（P〈0. 05 on 12 h,1 d,2 d and 3 d） and up-regulated the number of Bcl-2 positive cells（P〈0. 05 on 12 h, 1 d, 3 d, 5 d and 10 d）, and meanwhile down-regulated the number of Bax positive cells （P〈0. 05 on 3 h, 12 h, 1 d, 2 d, 3 d, 5 d and 10 d） in the ipsilateral hemisphere. Conclusion The neuroprotective effect of GRb1 on cerebral ischemia-reperfusion injury is related to inhibit neuronal apoptosis and to up-regulate the expression of Bcl-2 with down-regulating the expression of Bax.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2008年第2期214-217,共4页
Journal of Sichuan University(Medical Sciences)
基金
四川省教育厅重点项目(批准号2000-A28)资助
