摘要
目的探讨血管内皮生长因子(VEGF)及其受体Flk-1在糖尿病鼠肾脏中的表达变化及缬沙坦对其影响。方法建立糖尿病肾病(DN)大鼠模型,分为DN组、缬沙坦干预组和对照组,采用逆转录聚合酶链式反应技术(RT-PCR)、Western Blotting技术以及免疫组化技术检测VEGF和Flk-1的表达,检测尿蛋白、肾小球面积和体积,并分析数据相关性。结果DN组及缬沙坦干预组VEGF和Flk-1 mRNA和蛋白表达、尿蛋白、肾小球面积和体积均高于对照组(P<0.05),但缬沙坦干预组上述指标均低于DN组(P<0.01)。VEGF、Flk-1与尿蛋白、肾小球面积和体积呈正相关(P<0.05)。结论VEGF及其受体Flk-1在DN发病机制中起重要作用,过度表达导致肾脏损伤,血管紧张素受体拮抗剂缬沙坦具有通过抑制VEGF和Flk-1异常表达这一非血流动力学的肾脏保护作用。
Objective To explore effects of valsartan on expression changes of vascular endothelial growth factor （VEGF） and its receptor FIk-1 in diabetic rat kidney. Methods To establish the diabetic nephropathy （DN） rat models and divide the experiment rats into three groups-the DN group, the valsartan group and the control group, and use the reverse transcriptase polymerase chain reaction （RT-PCR）, Western Blotting and immunohistochemical techniques to detect the expression of VEGF and FIk-1, and detect the urine protein and glomerular area and volume, then analyze the relationship of the data. Results The mRNA and protein expression of VEGF and FIk-1, the urine protein and glomerular area and volume in the DN were higher than those in the control group and valsartan group （P〈0. 05）. VEGF and FIk-1 were positively correlated with the urine protein and glomerular area and volume （P〈0.05）. Conclusion VEGF and FIk-1 play an important role in the pathogenesis of DN, of which over-expression may lead to the damage of kidney. The angiotensin Ⅱ receptor antagonist- valsartan can protect kidney through the non-hemodynamic mechanism of inhibiting the abnormal expression of VEGF and FIk-1.
出处
《四川大学学报（医学版）》
CAS
CSCD
北大核心
2008年第2期223-226,230,共5页
Journal of Sichuan University(Medical Sciences)
关键词
糖尿病肾病
血管内皮生长因子
血管紧张素Ⅱ受体拮抗剂
Diabetic nephropathy Vascular endothelial growth factor Angiotensin Ⅱ receptor antagonists
