摘要
建立了易行、可靠的小鼠颈部异位心脏移植模型,探讨完善小鼠颈部异位心脏移植模型的建立和手术方式的改进。在显微镜下对受体手术,游离受体右侧静外静脉和颈总动脉。对供体,经下腔静脉注射肝素钠全身肝素化后,于低温下切取供心;采用供心无名动脉与受体颈总动脉端端间断吻合,供心肺动脉与受体颈外静脉端端吻合,进行小鼠颈部异位心脏移植。术后通过视诊和触诊检测供心心跳,病理学检查移植心排斥情况。共进行实验50例,其中同系移植25例,同种异基因移植25例。术后复跳率92%;7 d存活率90%,1例因术后失血过多于第1 d死亡,供心总缺血时间40 min。改进后的术式手术成功率高,术中暴露充分、血管处理简单、手术创伤小、污染机率小等,是简单、实用、可靠的手术方式。
To develop the operation methods of heterotopic cervical heart transplantation in mice, under microscopy , the right external jugular vein and the common carotid artery of the recipient were liberated . After heparinization all over the body of the donor , the heart was obtained in low temperature. The innominate artery of the donor heart and the fight common carotid artery of the recipient were anastomosed using the end-to-end interrupted suture technique. The same method was used in the connection between the pulmonary artery of the donor heart and the right external jugular vein of the recipient. The function of the graft was followed up daily by inspection and palpation of heart beats. Pathological examination was done to definite the transplantation rejection. The results showed that the total ischemic time of the graft was 40 , and the 7-day survival rate of the recipient was increased to 90% by using this technique. The advantage of this model lies in easy exposition, short duration of the operation, easy treatment of the blood vessel, low probability of contaminate and high rate of success, so deserves to use in experimental study widely.
出处
《科学技术与工程》
2008年第5期1156-1159,共4页
Science Technology and Engineering
基金
国家自然科学基金(30571803)资助
关键词
移植
心脏移植
小鼠
transplantation heart transplantion mouse
