摘要
目的:探讨BALB/c小鼠巨细胞病毒(MCMV)性心肌炎模型的特征。方法:60只4周龄BALB/c小鼠随机分成2组:实验组(36只,MCMV腹腔注射)和对照组(24只,3T3细胞裂解液腹腔注射)。在注射后3、7、14、21、28、35、42、49、56、63和70 d,记录心电图,测血清抗心肌β1受体抗体,分批处死小鼠行心肌病理、免疫组化和MCMV DNA检测。结果:实验组心肌炎累积发病25只(25/36,69.4%),死亡4只(4/36,11.1%);心肌炎急性期心肌呈现弥漫性炎性细胞浸润和灶性心肌细胞变性坏死,慢性期心肌间质散在炎性细胞浸润,急慢性期心肌炎病理积分均在2分或以下;免疫组化示急性期心肌IL-1β和TNF-α蛋白强阳性表达。实验组心律失常累计发生率达50.0%,可出现各种心律失常,急性期以窦性、房性心律失常和传导阻滞为主,慢性期以室性和房性心律失常为主。实验组3-7 d时心肌组织可检测到MCMV DNA片段,14-70 d时则不能检测到。实验组前5周血清抗β1受体抗体滴度为0,第6-10周明显升高;对照组前7周该抗体滴度为0,第8-10周轻微升高。结论:MCMV心肌炎并不严重,心肌细胞变性坏死具有局灶性和散在性特点,可出现各种心律失常;心肌炎早期病变和心律失常的发生可能与病毒感染直接损伤有关,慢性期病变和心律失常的发生则可能与抗β1受体抗体的作用有关。
AIM： To study characteristics of murine cytomegalovirus （MCMV） myocarditis in BALB/c mice. METHODS： 60 male BALB/c mice at age of 4 weeks were randomly divided into two groups： 36 in experiment group （injection of MCMV, group A） and 24 in control group （injection of 3T3 liquid, group B）. At 3,7,14,21,28,35, 42,49,56,63 and 70 days after injection, serum antibodies against myocardial β1 receptor were detected and electrocardiogram was recorded. Gene expression of MCMV was tested. Pathological and immunohistochemical examination were also taken. RESULTS： Total incidences of myocarditis were 69.4%. At acute period, the main pathological changes were myocyte swelling, necrosis and inflammatory cell infiltrating in a focal and dispersed fashion. At chronic period, inflammatory cells infiltrated dispersedly. There were strong expression of cytokine IL - 1β and TNF - α at acute period in immunohisto- chemistry . Total incidences of cardiac arrhythmias were about 50. 0% in group A. Most of cardiac arrhythmias were sinus arrhythmias, atrial arrhythmias and conduction black at the former 5 weeks, and were ventricular and atrial arrhythmias at the latter 5 weeks in group A. Gene of MCMV was detected at 3 -7 days and was undetectable from 14 to 70 days after virus infection in group A. The titers of serum anti - β1 receptor autoantibedy were zero at former 5 weeks and were significantly high at latter 5 weeks in group A,while they were zero from 1 to 7 weeks, but mild elevation from 8 to 10 weeks in group B. CONCLUSION： Myocarditis induced by MCMV is mild, all kinds of arrhythmias can occur. Pathological changes of myocardium and arrhythmias may be associated with MCMV infection at early period and with anti -β1 receptor autoantibody at chronic period.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2008年第3期466-469,共4页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.39970306)
