摘要
目的检测慢性粒细胞白血病（CML）病人smac和survivin mRNA的表达情况,以探讨其在CML病情进展及预后中的意义。方法应用半定量RT-PCR技术检测71例CML病人和10名健康者上述基因的表达。结果smac和survivin mRNA在CML病人的阳性表达率分别为90.1%、63.4%,均明显高于正常对照组（χ2=22.176、6.773,P〈0.01、0.05）,二者在CML的加速期、急变期的表达水平明显高于慢性期（t=10.708-16.725,P〈0.01）,且二者在CML病人的表达水平呈正相关（r=0.833,P〈0.01）。结论smac和survivin基因与CML的病情进展密切相关,是监测CML病情进展及判断预后的有价值的分子标记物。
Objective To detect the expressions of smac and survivin mRNA in chronic myeloid leukemia （CML） and explore their clinical significance. Methods Using semi-quantitative RT-PCR technique, expressions of smac and survivin mRNA were detected in 71 CML patients and 10 healthy adults. Results A positive rate of smac and survivin in the patients was 90. 1 and 63.4%, respectively, significantly higher than that of the controls （χ2 =22. 176,6. 773,P〈0. 01,0. 05）. Both smac and sur vivin expressions were much higher in accelerated phase and acute transformation phase than in chronic phase （t= 10. 708-16. 725, P〈0.01）. The expression level of smac and survivin showed positive correlation （r=0. 833,P〈0.01）. Conclusion The smac and survivin genes are closely related to the advancement and prognosis of CML, which are valuable molecular markers to be used for monitoring the disease.
出处
《青岛大学医学院学报》
CAS
2008年第1期51-53,共3页
Acta Academiae Medicinae Qingdao Universitatis
