摘要
目的：探讨以Walker256大鼠乳腺癌腹水瘤细胞制备大鼠胫骨骨癌痛模型，为骨癌痛机制和治疗研究提供有用的工具。方法：以Walker256乳腺癌细胞接种幼年雌性SD鼠腹腔制备腹水瘤细胞，将15μl腹水瘤细胞注入雌性成年SD鼠左侧胫骨骨髓腔制备骨癌痛模型；以注射加热灭活瘤细胞的SD鼠为假手术对照鼠，以正常鼠为正常对照鼠。造模后1-4周观察模型鼠自由行走痛评分、辐射热痛觉阈值[缩爪反应潜伏期（paw withdrawal latency，PWL）]和机械刺激诱发痛觉阈值[缩爪反应阈值（paw withdrawal threshold，PWT）]改变，并对痛觉行为改变明显的模型鼠进行影像学检测。结果：本方法制备模型的成瘤率为67．3％。模型鼠在造模后15d自由行走痛评分显著高于正常鼠和假手术鼠（P〈0．01）；模型鼠的模型后肢对热刺激痛觉阈值和对机械刺激诱发痛觉阈值分别在造模后21d和18d明显低于正常鼠和假手术鼠（P〈0．01），同时也明显低于模型肢对侧后肢（P〈0．05）。影像学结果显示，痛行为改变明显的模型鼠模型后肢胫骨骨质结构遭到明显破坏。结论：采用Walker256乳腺癌腹水瘤细胞可成功制备与人类骨癌痛体症相似的大鼠胫骨骨癌痛模型。
Objective： To establish a tibial cancer pain model with Walker 256 mammary gland carcinoma cell line （ CCL）, so as to provide a useful tool for further study of the mechanisms underlying cancer pain and its treatment. Methods： Walker 256 mammary gland carcinoma cells were injected into the cavum abdominis of infant rats to prepare ascitic tumor fluid. A total of 15μl ascitic tumor fluid was injected into the cavum medullare ossium of left tibia in adult SD rats to establish tibial cancer pain model. Thermoinactivated cells were injected into the adult SD rats （sham group） ; normal SD rats served as normal control group. One to 4 weeks after injection, walk-associated pain, radiation heat-associated pain threshold （ paw withdrawal latency, PWL） and mechanical pain threshold （ pwa withdrawal thresh- old, PWT） were all observed. And the bone damage in the models with pain behavior obviously changed was monitored by radiograph analysis. Results： Successful tumor forming was achieved in 67.3% of rats. Fifteen days after model establishment ,walk-associated pain score of the model group was obviously higher than that of the sham group and normal control group（ P 〈 0.01 ）. The PWL and PWT of the injected tibia in the model group were obviously lower than those of the normal control group and sham group at 21 d and 18 d after injection （ P 〈 0.01 ） ; meanwhile, they were also obviously lower than those of the hind limbs of the contralateral side in the model group （ P 〈 0.05 ）. Radiograph data showed that the injected tibias of models with obviously changed pain behavior was obviously damaged. Conclusion： The rats model of tibial cancer pain, similar to the human bone cancer pain, can be successfully established by Walker 256 mammary gland cells.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
2008年第1期41-45,共5页
Chinese Journal of Cancer Biotherapy
基金
国家自然科学基金资助项目(No30600584)~~
关键词
骨癌痛
大鼠模型
胫骨
乳腺癌细胞
行走痛
辐射热刺激痛
机械刺激痛
bone cancer pain
rat model
tibia
breast neoplasms cell
walk-associated pain
radiation heat-associated pain
mechanical pain
