摘要
通过对龙须草田间越冬存活率、低温半致死温度、2℃和7℃下叶绿素含量及过氧化物酶（POD），过氧化氢酶（CAT），超氧化物歧化酶（SOD）等保护酶活性的研究，探讨龙须草在低温胁迫下的生理生化变化．结果表明，12月至翌年1月刈割龙须草的安全越冬率不低于95％，显著高于11月刈割的；3～10cm的刈割高度对龙须草越冬没有影响；龙须草叶片细胞膜相对电导率随温度的下降而增加，低温半致死临界温度约为-3．5℃；与常温相比，叶绿素含量在7℃下变化不明显，2℃处理48h后显著下降，减少了53．0％．2℃处理24h的龙须草叶片POD和CAT活性最大，分别高于对照69．9％和90．3％％，而SOD活性变化不大．3种酶对低温胁迫的响应大小顺序为CAT，POD，SOD．复温10d后7℃低温时龙须草和对照无生长差异，2℃低温下的龙须草叶色发黄．
The experiment of cold hardiness of Eulaliopsis binata was conducted after winter mowing and livability rate, low temperature for 50% killed with EC test, chlorophyl content and antioxidant enzyme activities in the leaves were investigated under 2 ℃ and 7 ℃ low temperatures. The results showed that the high livability （〉95%） after mowing was obtained during the period from December to next January, which was significant higher than that of mowing in November. No difference was observed for livability rate with 3-10 cm mowing height over all mowing period. Relative EC （%） increased with temperature decreasing. The LTso was about -3.5 ℃ for Eulaliopsis binata. Compared with normal temperature, chlorophyll content was no significant change under 7 ℃. While chlorophyll content significantly descend about 53.0% after 48 h under 2 ℃. The activities of peroxidase （POD） and catalase （CAT） changed significantly increasing by about 69.9% and 90.3% after 24 h under 2-2. However superoxide dismutase （SOD） activity had little change. The order was： CAT〉POD〉SOD. When the temperature increased ten days late, there was no difference in Eulaliopsis binata growth between 7 ℃ and normal temperature treatment while the leaves turned yellow under 2 ℃.
出处
《湖南农业大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第1期18-21,共4页
Journal of Hunan Agricultural University(Natural Sciences)
基金
湖南省重大科技攻关项目(99KY202121)
关键词
龙须草
刈割
低温半致死温度
叶绿素
保护酶
Eulaliopsis binata
mowing
low temperature for 50% killed
chlorophyl
cell protective enzyme
