摘要
应用冰冻切片组织化学技术，以还原型尼克酰胺腺嘌呤二核苷酸磷酸-黄递酶特异性地确定一氧化氮合成酶（NOS）在小鸡耳蜗中存在，观察其分布情况，发现听毛细胞底部颗粒较集中，染色深，毛细胞周围亦有散在颗粒，基底乳头近端与远端染色强弱相似。阳性反应神经纤维连接毛细胞底部。螺旋神经节细胞胞浆中有大量的蓝色颗粒，细胞核无着色，这些细胞大小均匀，呈圆形或椭圆形，周围有多量阳性神经纤维，提示可能为神经性NOS。血管盖内皮细胞胞浆中酶活性较强。对这些分布特点和意义进行了讨论。
Nitric oxide (NO) is a new messenger molecule. This study describes the localization of NO synthase (NOS) in the chicken cochlea by means of NADPH-daiphorase (NADPH-d) histochemical stain. The results showed that the nerve ending at the base of hair cells, nerve fibers and ganglion cells were positive for NADPH-d activity. Ganglion cells reacted more strongly than the surrounding nerve fibers. Enzyme activity is localized within the cytoplasm of the ganglion cells. The shape of these cells seems to be round or oval, and uniform in size. It was suggested that NOS in chicken cochlea was the neuronal NOS (nNOS). Also, NOS activity existed in the endothelium cells of the tegmentum vasculosum. These characteristics and the significance of study were discussed.
出处
《临床耳鼻咽喉科杂志》
CSCD
1997年第10期457-459,共3页
Journal of Clinical Otorhinolaryngology
关键词
雏鸡
一氧化氮合成酶
耳蜗
组织化学
Chicken
Nitric oxide synthase
Cochlea
Histochemistry
