摘要
目的:探讨Y型电迷宫训练对全脑缺血大鼠学习记忆能力和海马组织CA1区突触素表达的影响。方法:90只SD大鼠随机等分为假手术组、对照组和训练组。对照组和训练组均采用改良Pulsinelli's4血管闭塞法建立全脑缺血大鼠模型,假手术组除不电凝双侧椎动脉、不夹闭双侧颈总动脉外,其余操作与对照组和训练组相同。术后7d以Y型电迷宫训练大鼠,分别在训练的7d、14d、21d应用Y型电迷宫检测大鼠的学习记忆能力;HE染色观察3组大鼠海马组织CA1区神经元形态学变化并计数正常神经元;免疫组织化学方法染色观察海马组织CA1区突触素的表达。结果:训练后7d、14d、21d,对照组大鼠错误反应次数、全天总反应时间、潜伏期与假手术组比较,差异有统计学意义(P均<0.05);训练组大鼠与对照组比较,3个指标间差异亦有统计学意义(P均<0.05)。训练组、对照组各时点大鼠海马组织CA1区正常神经元数目均低于假手术组(P均<0.05),且训练组21d高于对照组(P<0.05)。对照组大鼠各时点海马组织CA1区突触素表达与假手术组比较,差异有统计学意义(P均<0.05);且训练组大鼠14d、21d海马组织CA1区突触素的表达较对照组增多(P均<0.05)。结论:Y型电迷宫训练可以改善全脑缺血大鼠的学习记忆能力,其机制可能与促进海马组织CA1区突触素的表达有关。
Aim ： To explore the effects of Y-type maze training on the learning and memory abilities and the expression of Synaptophysin in hippocampus CA1 region in rats with global cerebral ischemia. Methods：A total of 90 SD rats were randomly divided into three groups：sham operation group, control group, and trained group. Global cerebral ischemic rat model was established by modified Pulsinelli＇s 4-vessel occlusion method in control group and trained group. Seven days after global cerebral ischemia, the rats were trained by Y-type maze. At the respective time point （7 d, 14 d, and 21 d） ,the cognitive ability was evaluated by Y-type maze. Hematoxylin-eosin staining was applied to observe neuronal pathomorphological changes and the number of normal neurons in hippocampus CA1 region. Immunhistochemical method and photo analysis were used to identify the expression of Synaptophysin in hippocampus CAI region. Results： After training for 7 d,14 d, and 21 d,the difference of cognitive ability among sham operation group, trained group, and control group was significent （ P 〈 0. 05 ）. The number of normal neuron in hippocampal CA1 of control group and trained group at three time points were significantly lower than those of sham operation group （ P 〈 0.05 ） , and the number of nurmal neuron of trained group at 21 d was higher than that of control group （P 〈 0.05 ）. There were significant difference on expression of Synaptophysin at three time points when control group compared with sham operation group （P 〈 0.05 ）. And at 14 d and 21 d, the expression was higher in trained group than those of control group （ P 〈 0.05 ）. Conclusion ： The training of Y-type maze could improve the learning and memory abilites in rats with global cerebral ischemia. Its mechanism is probably concerned improving Synaptophysin expression in hippocampus CA1 region.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2008年第2期342-345,共4页
Journal of Zhengzhou University(Medical Sciences)
关键词
全脑缺血
Y型电迷宫
突触素
学习记忆能力
大鼠
global cerebral ischemia
Y-type maze
Synaptophysin
learning and memory abilities
rat
