摘要
目的观察表皮生长因子（EGF）61＊G／A、转化生长因子-β1（TGF-β1）-509＊TIC、血管内皮生长因子（VEGF）936＊T／C基因功能多态性对术后结肠吻合口瘘的影响。方法采用聚合酶链反应-限制性片段长度多态性（PCR—RFLP）方法检测伴有和不伴有术后结肠吻合口瘘结直肠癌患者的EGF 61＊G／A，TGF-β1—509＊T／C，VEGF 936＊T／C基因型。结果伴有术后结肠吻合口瘘的结直肠癌患者TGF-β1-509＊T／T基因型比例（0．0％）低于无术后吻合口瘘的患者（15．2％），两者差异有统计学意义（P〈0．05）。伴有术后结肠吻合口瘘的VEGF 936＊C／C基因型和C等位基因比例（65．9％和80．6％）低于无术后吻合口瘘的患者（82．6％和90．5％），两者差异有统计学意义（基因型x^2=5．755，P〈0．05；等位基因x^2=5．275，P〈0．05）。伴有术后结肠吻合口瘘的结直肠癌患者EGF 61＊G／G基因型比例（25．0％）低于无术后吻合口瘘的患者（55．5％），但两者差异无统计学意义（x^2=1．591，P〉0．05）。结论TGF-β1-509＊T／T基因型，VEGF 936＊C／C基因型或936＊C等位基因有助于减少术后结肠吻合口瘘的发生。
Objective To understand the effect of EGF,TGF-β1 ,VEGF on the anastomotic leakage after colorectal cancer surgery by the investigation of EGF 61 ＊ G/A,TGF-β1 -509 ＊ T/C ,VEGF 936 ＊ T/C gene functional polymorphism. Methods PCR-RFLP was employed to study the EGF 61 ＊ G/A, TGF-β1 -509 ＊ T/C ,VEGF 936 ＊ T/C genotypes in colorectal cancer patients with or without anastomotic leakage after surgery. Results The frequency of TGF-β1 -509 ＊ T/T genotype in colorectal cancer patients with anastomotic leakage （0.0） was significantly lower than that in the patients without anastomotic leakage （ 13.2% ） （ P 〈 0.05 ）. The frequency of VEGF 936 ＊ C/C genotype and C allele in colorectal cancer patients with anastomotic leakage （63.9% and 80.6% respectively） was significantly lower than in the patients without anastomotic leakage （82.6% and 90.5% respectively） （genotypex2 = 5. 753 ,P 〈 0.05 ; allele x^2 = 5. 275, P 〈 0.05 ）. Although the EGF 61 ＊ G/G genotype in colorectal cancer patients with anastomotic leakage （25.0%） was less frequently found than in the patients without anastomotic leakage （ 35.5% ）, there was no statistically significant difference （x^2 = 1. 391 ,P 〉 0.05 ）. Conclusion TGF-β1 -509 ＊ T/T genotype, VEGF 936 ＊ C/C genotype and C allele decrease the risk of anastomotic leakage after surgery for colorectal cancer.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2008年第3期296-298,共3页
Chinese Journal of Experimental Surgery
关键词
结直肠肿瘤
基因多态性
吻合口瘘
Colorectal neoplasm
Genetic polymorphism
Anastomotic leakage
