摘要
采用高效液相色谱法能简单、快速和准确地同时测定联磺甲氧苄啶片中磺胺嘧啶（ＳＤ）、磺胺甲唑（ＳＭＺ）和甲氧苄胺嘧啶（ＴＭＰ）的含量。流动相为ｐＨ５．９０的０．０５ｍｏｌ／Ｌ磷酸盐缓冲液—甲醇（８０２０），使用ＯＤＳ柱能使片剂中的三组分与内标咖啡因达到很好的分离。检测波长为２４０ｎｍ。线性校正图谱所获得的浓度范围ＳＤ与ＳＭＺ均为２４～１２０ｍｇ／Ｌ，ＴＭＰ为９．６～４８ｍｇ／Ｌ；平均回收率±ＲＳＤ分别为（１００．０±０．３１）％、（１００．４±０．１８）％、（９９．１±０．８５）％，ｎ＝７。本法与中国药典（１９９５年版）方法测定结果基本一致。
A simple,rapid and precise method for simultaneous assay of sulfadiazine(SD),sulfamethoxazole(SMZ)and trimethoprim(TMP)tablets was developed by HPLC.Three components of tablet and internal standard(caffeine)were separated on a reversed-phase ODS column by using a mobile phase containing a mixture of methanol and 0 05mol/L phosphate buffer(pH5 90)(20∶80).Three components were detected at 240nm.Linear calibration graphs were obtained for SD,SMZ and TMP over the concentration ranges of 24～120mg/L,24～120mg/L and 9 6～48mg/L,respectively.The average recovery(n=7)was 100 0% with RSD=0 31% for SD,100 4% with RSD=0 18% for SMZ,99 1% with RSD=0 85% for TMP.The results of HPLC determination of SD,SMZ and TMP compare favorably with those obtained with the CP 1995 method.
出处
《药物分析杂志》
CAS
CSCD
北大核心
1997年第5期297-301,共5页
Chinese Journal of Pharmaceutical Analysis
关键词
联磺甲氧苄啶片
高效液相色谱
sulfadiazine,sulfamethoxazole and trimethoprim tablet,sulfadiazine,sulfamethoxazole,trimethoprim,HPLC
