摘要
根据鸭生长激素基因内含子3的序列设计引物,利用PCR-SSCP方法对籽鹅进行了单核苷酸多态性分析,并检测其多态性。结果表明,籽鹅生长激素基因内含子3长约364 bp,共发现了7个突变位点,分别为A160G、C167G、T264C突变,以及AA型个体在176、177位点G、A碱基缺失和BB型个体在231、232位点C、T碱基缺失。产生AA、AB和BB 3种基因型,其中BB基因型为优势基因型,且基因型频率表现为BB>AB>AA;卡方检验结果表明,基因型分布符合Hardy-Weinberg平衡(P>0.05)。不同基因型与体重和屠体性状的关联分析结果表明:BB基因型个体的7周龄体重、9周龄体重、10周龄体重、胸肌重、胸肌率及心脏重显著高于AA型个体(P<0.05);BB基因型个体8周龄体重显著高于AB型个体(P<0.05);在所分析的性状中,BB基因型个体的均值最大,AB型次之,AA型最小。
The single nucleotide polymorphism （SNP） of GH gene was investigated in Zi geese. The primers for intron 3 in GH gene were designed according to the homologous sequence of duck and the SNPs were detected by PCR-SSCP method. The results showed that the length of intron 3 of goose GH gene was about 364 bp, seven SNPs were detected, which were A160G, C167G and T264C, in addition, deletions were found at the base position 176, 177, 231 and 232, respectively, in the two kinds of homozygote. Three kinds of genotypes （AA, AB and BB） were detected and BB genotype was dominant in population, the frequency of genotypes were BB〉AB〉 AA. The results of Chi-Square test indicated that the frequencies of GH gene fit with Hardy-Weinberg equilibrium in Zi geese. The correlation between the genotypes and body weight and carcass traits of Zi geese showed that 7 weeks body weight, 9 weeks body weight, 10 weeks body weight, breast muscle weight, breast muscle rate and heart weight of Zi geese with BB genotype were significantly higher than those with AA genotype （P〈0. 05）, 8 weeks body weight with BB genotype were significantly higher than those with AB genotype （P〈0.05）. As the whole, the average values of these traits of the individuals with BB genotype were the highest, while those with AA genotype showed the lowest.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2008年第4期443-448,共6页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家科技支撑计划重大专项项目(2006BDA01A09)
国家科技支撑计划重点项目(2006BDA14B06)
江苏省属高校自然科学基础研究面上项目(07KJB230138)
