摘要
目的:观察冠心宁注射液对大鼠心肌缺血再灌注损伤的保护作用,探讨冠心宁抗心肌缺血再灌注损伤的保护机理。方法:雄性SD大鼠30只随机分成假手术组(C组)、缺血再灌注组(L/R组)、冠心宁治疗组(G组),建立大鼠在体心肌缺血再灌注模型:C组丝线穿过冠状动脉前降支但不结扎,I/R、G组通过结扎心脏左冠状动脉前降支40 min,再灌注60 min制作缺血再灌注损伤动物模型。缺血前30 min,G组经腹腔注射冠心宁注射液10.0ml·kg^(-1),余两组注射等量0.9%氯化钠注射液。测定再灌注60 min后心肌超氧化物岐化酶(SOD)活性及丙二醛(MDA)含量,同时取每组大鼠心肌检测梗死面积,电镜下观察缺血区心肌超微结构变化。结果:与L/R组相比,G组再灌注后60 min心肌MDA含量降低(P<0.01),SOD活性增高(P< 0.01),梗死面积较小(P<0.01),心肌超微结构受损较轻。结论:冠心宁注射液对心肌缺血再灌注损伤有明显的保护作用,其机制与其增强心肌抗氧化作用有关。
Objective： To investigate the protective effects of Ganxinning injection on myocardium against ischemia and reperfusion injury in rat and its underlying mechanism. Method： Thirty male SD rats were randomly divided into 3 groups ： control group （ group C, n = 10）, ischemia-reperfusion group （ group I/R, n = 10） and Ganxinning injection group （ group G, n = 10） ： In control group, the anterior descending branch of left coronary artery was exposed and a piece of silk thread was Blaced around the artery but untied. The ischemia and reperfusion injury models in I/R and G groups were made by temporary ligation Of the anterior descending branch of left coronary artery, ischemia lasted for 40 min and repefusion for 60 min. In G group, the Ganxinning injection （ 10 ml· kg^ -1 ） was intraperitoneally injected 30 min before ischemia. In C and I/R groups,normal saline （10 ml·kg ^-1 ） was intraperitoneally inject. At the end of 60 min repeffusion, the hearts were removied for determination for myocardial SOD activity and MDA content , at the same time the hearts were harvested for determination of myocardial infarct size and the hearts sections were cut to examine ultrastructure by electron microscopy. Result： After repeffused, myocardial MDA content was significantly lower in G group （P ：〈 0.01 ）, but the SOD activiy was higher（ P 〈 0.01 ）. Compared with of I/R group, the inffaret area was significantly smaller in G group（P〈 0.01 ）. Electron-microscopic examination showed that myocardium ultrastructure in G group was more slightly damaged than that in I/R group. Conclusion： Ganxinning injection has significantly protective effect on myocardial ischemia reperfusion. The underlying mechanism could be related to the enhancement of myocardial antioxidation.
出处
《中国药师》
CAS
2008年第4期380-382,共3页
China Pharmacist
