摘要
目的:探讨Genistein-磁性纳米微粒(Genistein- magnetic-nanopanicles,GEN-M-NPs)对胃癌细胞株SGC-7901生长及凋亡的影响.方法:利用化学共沉淀法制备GEN-M-NPs,透射电镜观察纳米微粒的形态,高效液相法测定包封率.以胃癌细胞株SGC-7901为实验对象,用四甲基偶氮唑蓝(MTT)比色法观察GEN-M-NPs和Genistein对其增殖活性的影响,采用流式细胞术检测细胞凋亡率,琼脂糖凝胶电泳观察DNA片段化改变情况.结果:GEN-M-NPs呈球形,粒径约105 nm,分布均匀,包封率约为10.3%.GEN-M-NPs对SGC-7901细胞生长有明显抑制作用,且呈剂量和时间依赖性(P<0.05).与单药Genistein作用组相比,GEN-M-NPs具有缓释性(86.04% vs 67.11%.P<0.05).流式细胞仪检测不同浓度GEN-M-NPs作用72 h后的细胞凋亡率分别是5.62%±2.04%(10μmol/L)、13.46%±2.02%(20μmol/L)、26.40%±3.84%(40μmol/L)、37.34%±4.68%(80μmol/L)、49.43%±8.29%(100μmol/L),阴性对照组的凋亡率为2.60%±1.34%.DNA凝胶电泳结果显示40μmol/L组作用24 h可见明显的凋亡细胞形成的梯状条带.结论:GEN-M-NPs在体外能有效地抑制胃癌细胞增殖并诱导细胞凋亡,与Genistein相比,有缓释性.
AIM： To observe the effects of different concentrations of genistein-magneticnanoparticles （GEN-M-NPs） on the growth and apoptosis of gastric cancer cell line SGC-7901.
METHODS： GEN-M-NPs were prepared by chemical co-precipitation. Morphology of GEN- M-NPs was observed under a transmission electron microscope. Encapsulating efficiency of genistein was determined by high-performance liquid chromatography. After SGC-7901 cells were treated with various concentrations of GEN-M-NPs and genistein, their anti-tumor effects were determined by methyl thiazolyl tetrazolium （MTT） assay. Apoptosis rate was quantified by flow cytometry and DNA frag-mentation was evaluated by agarose gel electrophoresis.
RESULTS： GEN-M-NPs were round or elliptical in shape with a diameter of about 105 nm. Their encapsulating efficiency was about 10.3%. The proliferation of SGC-7901 cells in vitro was significantly inhibited by GEN-M-NPs in a time- and dose-dependent manner （P 〈 0.05）. The release of GEN-M-NPs was slower than that of genistein （86.04% vs 67.11%, P 〈 0.05）. After treatment with various concentrations of GEN- M-NPs for 72 h, the apoptosis rate for GEN-M- NPs and negative control groups was 5.62% ± 2.04% （10 μmol/L）, 13.46% ± 2.02% （20μmol/L）, 26.40% ± 3.84% （40 μmol/L）, 37.34% ± 4.68%（80 timol/L）, 49.43% ± 8.29% （100 timol/L）, and 2.60% ± 1.34% （control）, respectively. Agarose gel electrophoresis showed typical apoptotic DNA ladders 24 h after treatment with 40 μmol/L GEN-M-NPs.
CONCLUSION： GEN-M-NPs can effectively inhibit the growth and apoptosis of SGC-7901 cells.
出处
《世界华人消化杂志》
CAS
北大核心
2008年第9期998-1003,共6页
World Chinese Journal of Digestology
关键词
染料木黄酮
胃癌细胞
凋亡
纳米微粒
四甲基偶氮唑蓝
Genistein
Gastric cancer
Apoptosis
Nanoparticles
Methyl thiazoly tetrazolium assay
