摘要
目的研究骨髓间充质干细胞(MSCs)对皮肤光老化的修复作用,为皮肤光老化的治疗提供新途径。方法每天UV-A和UV-B紫外线灯同时照射2 h,连续照射50 d,制备豚鼠皮肤光老化模型;体外分离培养豚鼠乳鼠MSCs;经DAPI标记后,局部多点皮内注入豚鼠皮肤紫外线照射区域,激光共聚焦显微镜下分别观察注入后24 h、5 d及10 d,DAPI标记的MSCs在紫外线照射区域皮肤组织的迁徙及定居;肉眼及HE染色切片观察MSCs对豚鼠光老化皮肤的修复作用。结果DAPI标记的MSCs经紫外线照射皮肤区域局部皮内多点注射24 h后,即出现在真皮层,5 d后出现在真皮层及毛囊组织,10 d后广泛弥散在皮肤各层。肉眼及皮肤HE染色切片镜下观察均显示,治疗组豚鼠较模型组明显延缓光老化进程,且在停止照射后,治疗组光老化症状比自然恢复组有更为显著的改善。结论MSCs对皮肤光老化有明显的延缓及修复作用。
Objective To explore the repair of skin photoaging by bone marrew-derived mesenchymal stem cells （MSCs） and provide a novel route for the treatment of skin photoaging. Methods Establish guinea pig model of skin photoaging by simultaneous irradiation with UV-A and UV-B ultraviolet radiators for 50 d,2 h per day.The MSCs of suckhng KM mice were isolated and cultured in vitro,then labeled with DAPI and injected i.d. into the irradiation region of model guinea pigs. Observe the migration and colonization of MSCs in irradiation region by laser scanning confocal microscopy 24 h,5 d and 10 d after injection,and the repair of skin photoaging by macroscopic examination and HE staining.Results MSCs were observed in epidermis 24 h,in both epidermis and hair follicle tissue 5 d and diffused to all cortical layers 10 d after injection. Both macroscopic examination and HE staining showed that, compared with those of control, the skin photoaging of guinea pigs treated with MSCs was significantly delayed,and improved significantly after stopping irradiation. Conclusion delayed and repaired skin photoaging significantly.
出处
《中国生物制品学杂志》
CAS
CSCD
2008年第4期315-317,共3页
Chinese Journal of Biologicals
基金
吉林省科技厅基金项目(200705205)
关键词
骨髓间充质干细胞
光老化
迁徙
定居
修复
Bone marrow-derived mesenchymal stem cells
Photoaging
Migration
Colonization
Repair
