摘要
目的探讨发生在下肢骨骼肌的缺血预适应对肢体缺血再灌注(LIR)后心肌的作用。方法Wistar大鼠24只随机分为对照组(C)、缺血再灌注组(I/R)和缺血预适应组(IPC+I/R)。测定心肌组织XO、SOD和MDA含量;检测血浆CK、CK-MB及心肌组织MPO水平;检测心肌组织Bcl-2、Bax蛋白表达情况;光镜下观察心肌组织的形态学变化。结果LIR后心肌组织SOD活性降低,MDA、XO含量增加(P<0.01或P<0.05),心肌组织MPO及血浆CK、CK-MB水平均较C组明显升高(P<0.01)。Bcl-2和Bax蛋白表达增多,但Bcl-2/Bax比值降低。镜下可见心肌细胞水肿等组织损伤的征象;在IPC+I/R组,心肌组织SOD的减少和MDA、XO及MPO含量的增加受到一定程度抑制,血浆CK、CK-MB水平有所下降(P<0.01),Bcl-2表达增强而Bax表达减少,Bcl-2/Bax比值升高(P<0.01)。光镜下心肌组织的形态学表现也有所改善。结论大鼠骨骼肌缺血预适应可能通过抗氧化、抑制炎性反应和细胞凋亡等途径发挥对肢体缺血再灌注后心肌的远端保护效应。
Objective To investigate the effect of ischemic preconditioning on myocardium in rats which suffer from limb ischemiamperfusion. Methods 24 Wistar rats were randomly divided into 3 groups：control group （C）, ischemia-mperfusion group （I/R） and ischemic preconditioning group （IPC＋I/R）. The contents of xanthine oxidase （ XO ）, superoxide dismutase （SOD）and malondialdehyde （MDA） in myocardial tissues were determined in different groups. The activities of cmatine kinase（CK） and creatine kinase MB isoenzyme（CK-MB） in plasma,the level of myelopemxidase （MPO） in myocardium and the expression of Bcl-2 and Bax were measured . The morphologic changes of myocardium were observed under the microscope. Results After ischemia-mperfusion of rats＇ limbs, the level of SOD in myocardium decreased,while the contents of XO and MDA and MPO increased to different degrees （P 〈 0.01 or P 〈 0.05）. The values of CK and CK- MB in plasma increased obviously, the expression of Bcl-2 and Bax was stronger, and the ratio of Bcl-2/Bax was lower than those in the control group,all with significant differences. Under the microscope,myocardium injury signs were seen in some areas. However in IPC ＋ I/R group,values of the injury indexes were all decreased,and IPC prevented myocardium from oxidation,inflammation and apoptosis. Conclusion Skeletal muscle ischemic preconditioning can protect the myocardium during limb ischemia-reperfusion,and, the protection might be related to anti-oxidation,anti-inflammation and inhibiting apoptosis.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2008年第2期168-170,共3页
Journal of China Medical University
基金
河北省教育厅科研基金资助项目(2001135)
关键词
缺血预适应
再灌注损伤
心肌
ischemic preconditioning
reperfusion injury
myocardium
