期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

PCR-SSCP检测肺癌EGFR基因突变 被引量：2

Study on PCR-SSCP Detecting EGFR Mutations in Lung Cancer
在线阅读 下载PDF
导出
摘要 目的分析PCR-SSCP检测肺癌EGFR基因突变的敏感性。方法应用PCR-SSCP检测36例肺癌标本的突变情况并与其它文献进行比较。结果在36例肺癌标本中,有11例存在突变,突变率为30.6%(11/36),其中男性2例,女性9例。结论PCR-SSCP检测肺癌EGFR基因突变具有较高的敏感性,与其它文献报道一致。 Objective To analyse sensitivity of PCR-SSCP on detecting EGFR gene mutations in lung cancers. Methods PCR-SSCP was employed to detect EGFR mutation in 36 samples with lung cancer. Then the mutation rates were calculated and compared with other relevant to documents. Results 11 of 36 lung cancer cases yielded the EGFR mutations,of which,2 were males and 9 were females. Conclusion It is sensitive for PCR-SSCP to detect EGFR mutation in lung can-cers.
作者 奉水东 陈敏时 陈新 让蔚清 凌宏艳
机构地区 南华大学流行病学教研室 南华大学卫生毒理学教研室 南华大学生理学教研室
出处 《现代检验医学杂志》 CAS 2008年第2期13-15,共3页 Journal of Modern Laboratory Medicine
基金 湖南省卫生厅资助项目(B200695)
关键词 聚合酶链反应 单链构象多态性 肺癌 表皮生长因子受体基因 检测 突变 PCR-SSCP lung cancer EGFR gene detection mutation
分类号 R734.2 [医药卫生—肿瘤] Q754 [生物学—分子生物学]
  • 相关文献

参考文献13

  • 1Paez JG ,Janne PA ,Lee J C ,et al. EGFR mutations in lung cancer :correlation with clinical response to gefitinib therapy[J]. Science, 2004, 304 (5676): 1497- 1500.
  • 2Lynch TJ,Bell DW,Sordella R,et al. Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small-cell lung cancer to gefitinib[J]. N Engl J Med, 2004,350(21):2129- 2139.
  • 3周彩存,周崧雯,潘虹,粟波,高志强.TaqMan—MGB探针实时荧光聚合酶链反应快速检测非小细胞肺癌表皮生长因子受体基因突变[J].中华肿瘤杂志,2007,29(2):119-123. 被引量：11
  • 4周冬辰,李龙芸,崔全才,王孟昭,张力,穆新林.变性高效液相色谱法分析中国人非小细胞肺癌、结直肠癌中表皮生长因子受体突变情况和临床意义[J].癌症进展,2006,4(5):462-466. 被引量：5
  • 5董强刚,韩宝惠,黄进肃,杨立民,黄建,赵春英,卢丽琴.176例非小细胞肺癌的EGFR基因突变分析[J].中华肿瘤杂志,2006,28(9):686-690. 被引量：53
  • 6Fukuoka M ,Yano S,Giaccone G ,et al. Multi-institutional randomized phase Ⅱ trial of gefitinib for previously treated patients with advanced non-small-cell Lung cancer[J]. J Clin Oncol, 2003, 21 (12) : 2237- 2245.
  • 7Kris MG,Natale RB,Roy S Herbst,et al. Efficacy of gefitinib,an inhibitor of the epidermal growth factor receptor tyrosine kinase, in symptomatic patients with non-small cell lung cancer:A randomized trial [J]. JAMA, 2003 ; 290(16) : 2149-2158.
  • 8刘峰,姜斌,龚圣济,姚宝娣,张文颖,朱冠山,朱忠政,龚玉芳,王美玲,胡晓华.中国人非小细胞肺癌EGFR和K-RAS基因突变情况的研究[J].中华医学遗传学杂志,2007,24(1):31-34. 被引量：13
  • 9Asano H, Toyooka S,Tokumo M,et al. Detection of EGFR gene mutation in lung cancer by mutant-enriched polymerase chain reaction assay[J]. Clin Cancer Res, 2006,12 (1) : 43-48.
  • 10Nagai Y ,Miyazawa H, Qun H ,et al. Genetic Heterogeneity of the epidermal growth factor receptor in non-small cell lung cancer cell Lines revealed by a rapid and sensitive detection system,the peptide nucleic acid-locked nucleic acid PCR clamp[J]. Cancer Res, 2005,65 (16) : 7276-7282.

二级参考文献65

  • 1BaoMingQIN,XiaoCHEN,JingDeZHU,DuanQingPEI.Identification of EGFR kinase domain mutations among lung cancer patients in China:implication for targeted cancer therapy[J].Cell Research,2005,15(3):212-217. 被引量：67
  • 2管忠震,张力,李龙芸,蒋国梁,刘叙仪,储大同,赵洪云,李玮.吉非替尼治疗局部晚期或转移性非小细胞肺癌在中国的临床研究[J].癌症,2005,24(8):980-984. 被引量：114
  • 3[1]Parkin DM.Global cancer statistics in the year 2000.Lancet Oncol,2001,2 (9):533
  • 4[2]Lynch TJ,Bell DW,Sordella R,et al.Activating mutations in the epidermal growth factor receptor underlying responsiveness of non-small cell lung cancer to gefitinib.N Engl J Med,2004,350 (21):2129
  • 5[3]Paez JG,Janne PA,Lee JC,et al.EGFR mutations in lung cancer:Correlation with clinical response to gefitinib therapy.Science,2004,304 (5676):1497
  • 6[4]Herbst RS,Maddox AM,Rothenberg ML,et al.Selective oral epidermal growth factor receptor tyrosine kinase inhibitor ZD1839 is generally well-tolerated and has activity in non-small cell lung cancer and other solid tumors:Results of a phase Ⅰ trial.J Clin Oncol,2002,20 (18):3815
  • 7[5]Nagahara H,Mimori K,Ohta M,et al.Somatic mutations of epidermal growth factor receptor in colorectal carcinoma.Clin Cancer Res,2005,11 (4):1368
  • 8[6]Bardelli A,Parsons DW,Silliman N,et al.Mutational analysis of the tyrosine kinome in colorectal cancers.Science,2003,300 (5621):949
  • 9[7]Shigematsu H,Lin L,Takahashi T,et al.Clinical and biological features associated with epidermal growth factor receptor gene mutations in lung cancers.J Natl Cancer Inst,2005,97 (5):339
  • 10[8]O'Donovan M,Oefner P,Roberts S,et al.Blind analysis of denaturing high-performance liquid chromatography as a tool for mutation detection.Genomics,1998,5244 (1):44

共引文献113

同被引文献27

  • 1董强刚,韩宝惠,黄进肃,杨立民,黄建,赵春英,卢丽琴.176例非小细胞肺癌的EGFR基因突变分析[J].中华肿瘤杂志,2006,28(9):686-690. 被引量：53
  • 2Franklin WA. Veve R, Hirsch FR, et al. Epidermal growth factor receptor family in lung cancer and pre?malignancy[J]. Semin Oncol, 2002,29 (1 suppl 4): 3- 14.
  • 3Cohen S. Purification of the receptor for epidermal growth factor from A-431 cells: its function as a ty?rosyl kinase[J]. Methods Enzymol, 1983 (99): 379- 387.
  • 4Kosaka T, Yatabe Y , Endoh H. et al. Mutations of the epidermal growth factor receptor gene in lung cancer: biological and clinical implications[J]. Cancer Res, 2004,64(24):8919-8923.
  • 5Lynch T],Bell DW,Sordella R,et al. Activating mu?tations in the epidermal growth factor receptor under?lying responsiveness of non-small-cell lung cancer to gefitinib[J]. N EnglJ Med,2004. 350(21): 2129- 2139.
  • 6Mitsudomi T, Yatabe Y. Mutations of the epidermal growth factor receptor gene and related genes as de?terminants of epidermal growth factor receptor tyro?sine kinase inhibitors sensitivity in lung cancer[J]. Cancer Sci, 2007,98(12) : 1817-1824.
  • 7Toyooka S, Yatabe Y. Tokumo Mver al. Mutations of epidermal growth factor receptor and K-ras genes in adenosquamous carcinoma of the lung[J]. Int] Cane- er,2006,118(6):1588-1590.
  • 8Tatematsu A. Shimizu], Murakami Y, et al. Epider?mal growth factor receptor mutations in small cell lung cancer[J]. Clin Cancer Res, 2008,14(19): 6092- 6096.
  • 9Mok T, Wu YL, Thongprassert S, et al. Phase III. randomized, open-label, first-line study of gefitinib vs catboplatiri/ paclitaxel in clinically selected patients with advanced non-small cell lung cancer (ESMO 2008,LBA2)[J]. Ann Oncol,2008,19(suppI8): VJH.
  • 10Mitsudomi T,Morita S, Yatabe Y,et al. Gefitinib versus cisplatin plus docetaxel in patients with non?small-cell lung cancer harbouring mutations of the epidermal growth factor receptor (W]TOG3405): an open label, randomised phase 3 trial[J]. Lancet Oncol,2010, 11(2): 121-128.

引证文献2

二级引证文献13

现代检验医学杂志
2008年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部