摘要
研制一种NO传感针并将其应用归经实验的在体测量中。以壳聚糖与Ni^2＋形成的配合物作为敏感膜，浸涂在微铂丝制成的基体电极上，再修饰Nation膜层；在中医穴位归经实验中，用此传感针测定小鼠穴位和穴位旁开的两检测点在刺激前后的NO浓度。测定结果显示，线性范围为10^-6-10^-4mol／L，R^2=0．9848；重复性误差为4．72％；平均回收率为97．74％；归经实验中，NO电流值在刺激前后穴位都明显高于穴位旁开时（P〈0．05）该NO传感针的选择性好、稳定性高、重复性好，其针状结构可适用于在体测量。在体实验结果表明：存在穴位特异性，并证实了经脉的循经性，为循经传感理论提供了实际的技术支持。
A kind of NO sensor is developed, and it is used in the vivo measurement of meridian experiments. The nitric oxide sensor is made of the pure platinum wire modified by the chitosan and Ni^2＋ solution and decorated by the Nation membranes from its surface. This sensor is used to measure the nitric oxide concentration of the acupoints and non-acupoints before and after acupuncture in the rats. The linear response range of this sensor is 10^-6 - 10^-4 mol/L with the square of the correlation coefficient 0. 984 8. Reproducibility is 4.72 %, average recovery is 97.74 %, in the experiments of meridian, the current of NO in the acupoints is obviously higher than in the non-acupoints （P 〈0.05）. Its selectivity, stability and repeatability are good and its pintype structure is applicable to vivo measurement. The results in vivo experiments of the rats show that there is the specificity in the acupoints and suggest that there is the existence of meridian specificity ,so this sensor can provide a support in the technology for the theory of the meridian and acupoint.
出处
《传感器与微系统》
CSCD
北大核心
2008年第4期86-88,91,共4页
Transducer and Microsystem Technologies
基金
国家自然科学基金重大研究计划资助项目(90209009)
关键词
NO
传感针
经络
穴位
nitric oxide
sensor
meridian
acupoint
