摘要
目的:研究CD147对白血病细胞U937生长和肿瘤形成的影响。方法:分别采用脂多糖(LPS)或CD147单克隆抗体处理U937细胞;用RT-PCR和流式细胞术分别在mRNA和蛋白水平检测各组中CD147的表达情况;用流式细胞术检测在LPS和CD147单克隆抗体作用下U937细胞周期的变化;用MTT法对各组细胞的生长状况进行分析;将细胞经皮下接种于裸鼠体内,对各组间肿瘤生长速度、肿瘤体积及裸鼠存活时间进行统计分析。结果:LPS在体外能够诱导白血病细胞U937表面CD147的表达,同时细胞增殖旺盛,但细胞凋亡数增加;使用CD147抗体阻断CD147后,能够将细胞周期阻断在G0/G1期,细胞活力下降,并诱导细胞凋亡;CD147抗体体外预处理能够抑制U937细胞在裸鼠体内的生长,使小鼠存活时间延长。结论:LPS可诱导U937细胞表面CD147分子表达增加,从而促进U937细胞的生长和肿瘤形成。
Objective： To investigate the effects of CD147 on proliferation and tumorigenicity of leukemia cells U937. Methods： U937 cells were treated by lipopolysaccharide（LPS） or anti-CD147 monoclonal antibody, and the expression of CD147, the cell cycle as well as the cell viability and the growth of the implanted tumors in nude mice were investigated. Results： Expression of CD147 was elevated by the induction of LPS. The cell cycle analysis showed that the proportions of S-phase cells were increased by LPS induction, and CD147 antibody could cause the cells arrest at G0/G1 phase and undergo apoptosis. The cell viability decreased by anti-CD147 antibody treatment. Implantation of anti-CD147 antibody pretreated U937 cells to the nude mice by subcutaneous injection showed a significant delay of tumor appearance, a better animal survival and a remarkable reduction of tumor volume. Conclusion： Overexpression of CD147 on U937 cells may increase proliferation of U937 and promote the tumorigenicity of the cells in nude mice.
出处
《生物技术通讯》
CAS
2008年第2期203-206,共4页
Letters in Biotechnology
