摘要
用设计的特异性引物,通过RT-PCR法对1999和2004年间分离自山东省的具有一定代表性的3株鸡新城疫病毒（ShD-2-04,ShD-3-99,ShD-5-04）进行HN基因的扩增和克隆,并对其进行核苷酸序列测定和分析。结果表明：3个毒株HN基因开放性阅读框架（ORF）均为1716bp,编码571个氨基酸,属强毒的C群;3株山东分离毒的核苷酸同源性为98.2%-98.8%,氨基酸同源性为98.4%-98.8%,其中ShD-2-04和ShD-5-04核苷酸及氨基酸同源性均为最高,达到99.8%和99.8%;将3株山东分离毒株与国内外标准强毒株及弱毒株的HN基因核苷酸及氨基酸同源性分别进行比较：3毒株与国内外标准毒株HN的核苷酸同源性为82.7%-87.2%,氨基酸同源性为87.6%-90.0%,表明已发生一定的变异。
With a pair of specific primers, the fragment containing hemagglutinnin-neuriminidase （HN） genes of three representative strains of Newcastle disease virus （ NDV ） , ShD-2-04, SHD-3-99 and ShD-5-04, isolated from Shandong between 1999 and 2004 were amplified by reverse transcription -polymerase chain reaction （RT-PCR）. The HN genes were sequenced after PCR products were cloned. The results showed that all the three Shandong isolates had opening reading fragment （ORF） with the length of 1 716 bp, encoding 571 amino acids and belonged to C virulent group. The homology percentages of nucleotides and amino acids sequences were 98. 2% - 98. 8% and 98. 4% - 98.8%, respectively. The strain ShD-2-04 and ShD-5-04 displayed the highest homologies of nucleotides （98.8%） and amino acids （98. 8% ）. Compared with HN gene sequences of the published strains, the homology percentages of nueleotides and amino acids sequences were 82. 7% -87. 2% and 87.6% -90. 0% , respectively.
出处
《畜牧与兽医》
北大核心
2008年第2期20-23,共4页
Animal Husbandry & Veterinary Medicine
基金
山东省财政支持项目(SDGP2004-54-O)
关键词
新城疫病毒
HN基因
序列分析
Newcastle disease virus
HN gene
sequence analysis
