摘要
研究了以聚乙烯醇（PNA）为载体，固定化枯草杆菌．用于生产α-淀粉酶的方法。探讨了固定化条件对PVA固定化细胞结构、机械强度和产酶性能的影响，并与海藻酸钠固定化细胞以及游离细胞进行了比较。结果表明；PVA能有效地固定枯草杆菌细胞，进行正常的增殖和产酶，在同样条件下，这种固定化细胞的产酶能力比游离细胞高28％，比海藻酸钠固定比细胞高11％．
The method of immobilization of Bacillus subtilis by using polyvingyl alcohol(PVA) as the carrier of immobilized cells was studied. The effects of conditions of immobilization on the structure and meckanical strength of PVA immobilized cells and the ability for producing α-amylase of the immobilized cells were investigat-ed. The comparative test of fermentation by using the immbilized cells with PVA as carrier and the immobilized cells with sodium glyinate as carrier and free cells was carried out in the same condition. The result8 showed that Bacillus substilis cells were immobilized efficently by PVA and the immobilized cells could normally grow and re-produce and produce α-amylase. In the same condition .the activity of α-amylase produced boy the immobilized cells was 28K higher than that produced by free cells and 11% higher than that produced by the cells immobilized with sedium glyinate as carrier
出处
《生物医学工程学杂志》
EI
CAS
CSCD
北大核心
1997年第4期359-362,共4页
Journal of Biomedical Engineering
