摘要
目的评价日本血吸虫重组蛋白(reSj16)调节宿主炎症反应的效果。方法在大肠埃希菌中对重组pGEX-4T-1-Sj16基因进行原核表达并纯化重组蛋白。56只昆明小鼠随机分为7组(每组8只),其中5个实验组,所用reSj16蛋白剂量分别为0.1、0.5、1.0、5.0和10.0μg/kg;并设地塞米松(5.0μg/kg)和生理盐水(0.1ml/只)对照组。进行小鼠耳廓肿胀和毛细血管通透性实验,观察reSj16蛋白对二甲苯致小鼠耳廓肿胀作用和毛细血管通透性改变的影响。56只SD大鼠进行角叉菜胶所致大鼠足跖肿胀实验,56只昆明小鼠进行实验性腹膜炎小鼠腹腔毛细血管通透性改变实验,观察reSj16调节宿主炎症反应效果。结果0.1、0.5、1.0、5.0和10.0μg/kg的reSj16蛋白均可明显减轻二甲苯所致小鼠耳廓肿胀和毛细血管通透性增加,抑制角叉菜胶所致大鼠足跖肿胀,明显抑制实验性腹膜炎小鼠腹腔毛细血管通透性增高,且具有一定的剂量依赖关系。1.0、5.0和10.0μg/kg的reSj16蛋白组与对照组相比,差异具有统计学意义。结论Sj16具有明显的调节宿主炎症反应的作用。
Objective To study the immunomodulation effect of the recombinationt Sj16 from Schistosoma japonicum（reSj16） on inflammation response of host. Methods reSj16 expressed in pGEX-4T-1 was purified by GST purification kit and detected by sodium dodecyl sulfate-polyacrylamide gel electropheresis （SDS-PAGE） and mass spectra （MS）. The immunomodulation effect of reSj16 was observed by means of dimethylbenzene inducing mouse ear edema model, carrageenan inducing rat voix pedis swell model and acetic acid inducing mouse experiment peritonitis model. Results The soluble protein of reSj16 was obtained and identified by SDS-PAGE and MS. reSj16 1.0, 5.0, 10.0 μg/kg evidently suppressed the mouse ear edema induced by dimethyl-benzene, significantly mitigated the rat voix pedis swelling induced by carrageenan, and remarkably suppressed the incr-ease of the capillary permeability of abdominal cavity in experiment peritonitis mouse model. Conclusion The results further prove that Sj16 may be a potential immunosuppressive molecule and may have a notable effect on immunomo-dulation.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2008年第2期113-118,共6页
Chinese Journal of Parasitology and Parasitic Diseases
基金
广州市科技计划项目(No.031727)
教育部博士点基金(No.[2005]216号)
高等学校博士学科点专项科研基金(No.20070558221)~~
