摘要
建立了用1,3-环己二酮-甲醛作为荧光衍生试剂测定氨苄西林钠的方法。氨苄西林钠与1,3-环己二酮-甲醛反应生成一种强荧光物质。其最大激发波长是336 nm,最大发射波长是420 nm。氨苄西林钠浓度在0.01～0.6μg/mL范围内与相对荧光强度呈线性关系,线性方程为ρ=0.0007931F-0.06133,线性相关系数为0.9998,相对标准偏差为1.8%,检出限为10 ng/mL,回收率为97.0%～105.9%。考察了pH、温度、放置时间、干扰离子等对测定的影响。此法可用于注射液中氨苄西林钠的测定。
Fluorimetric determination of ampicillin sodium is described. The ampicillin sodium reacts with 1,3-cyclo- hexanediono and formaldehyde to form a compound which is a strong fluorescent substance. The measurement was carried out at 420 um with excitation at 336 urn. The linear relationship was obtained between the relative fluorescence intensity and the concentration of ampicillin sodium in the range of 0.01 - 0.6 μg/mL. The linear regression equation of the calibration graph is C = 0.0007931F - 0. 06133 （ C is concentration of ampicillin sodium （μg/mL） and F is relative fluorescence intensity in the equation）, with a correlation coefficient of 0. 9998 and the relative standard deviation of 1.8%. The detection limit is 10 ng/mL, and the recoveries are from 97.0 to 105.9%. This method can be used for the determination of ampicillin sodium in injection samples.
出处
《分析试验室》
CAS
CSCD
北大核心
2008年第5期42-45,共4页
Chinese Journal of Analysis Laboratory
基金
山东省自然科学基金(Y2006B31)项目资助
