摘要
以矮化梨(Pyrus communis L.)与茌梨(P.bretschneideri Rehd.)的F1杂交分离群体共110个单株(3a生,矮化型和正常型各55株)为试材,对来自西洋梨的矮化型突变基因pcDw进行了SSR分子标记研究。用分离群体分组分析法(Bulked Segregant Analysis,BSA),通过对源自梨、苹果和桃基因组的共40对SSR(Simple Sequence Repeat)引物的筛选,获得了一个与pcDw基因连锁距离为9.3cM的SSR标记KA14210,由此将该基因定位到了梨品种Barlett遗传图谱的第16连锁群上。
The F1 segregation population, total of 110 （3-year-old） seedlings derived from the cross Aihuali （Pyrus communis L.） ×Chili （P. bretschneideri Rehd.） was tested for identifying SSR molecular markers linked to a pear dwarf gene pcDw, which produced by a mutation of P. communis. Altogether, 40 pairs of SSR primers originating from pear, apple and peach genome were screened by bulked segregant analysis （BSA）. Finally, a SSR marker, KA14210, which linked topcDw gene at a distance of 9.3 cM, was acquired. By this marker, pcDw gene was located on LG16 of the genetic map of pear variety Barlett.
出处
《果树学报》
CAS
CSCD
北大核心
2008年第3期404-407,共4页
Journal of Fruit Science
基金
国家"863"计划(2006AA100108-3-5)
山东省自然基金(Y2003D01)
青岛市科技攻关(05-2-NS-18)
