摘要
目的:观察丝瓜(LC)多酚的抗氧化作用及丝瓜对实验性高脂血症小鼠的影响。方法:利用丙酮提取丝瓜多酚类物质,用福林法测定其含量并观察丝瓜多酚粗提液抑制羟自由基生成的能力。用高脂饲料喂养昆明小鼠建立高脂血症模型,以血脂康作为阳性对照观察饲料中补充丝瓜冻干品喂养对小鼠血清胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、超氧化物歧化酶(SOD)及肝组织中丙二醛(MDA)的影响。结果:(1)新鲜丝瓜多酚含量为0.3g/kg±0.1g/kg。(2)新鲜丝瓜多酚粗提液具有较强的抑制羟自由基生成的能力。(3)与高脂模型组比较,低剂量丝瓜组、高剂量丝瓜组小鼠体重增加趋势明显减轻。(4)低剂量丝瓜组TC(4.19±0.37)mmol/L和LDL-C(2.77±0.79)mmol/L、高剂量丝瓜组TC(3.56±0.55)mmol/L和LDL(2.34±0.41)mmol/L、血脂康阳性对照组TC(4.59±0.96)mmol/L和LDL-C(3.25±0.67)mmol/L均低于高脂模型组TC(7.20±0.87)mmol/L和LDL-C(4.92±0.52)mmol/L(P<0.01),其中高剂量丝瓜组TC、LDL-C下降最为明显。(5)低剂量丝瓜组和高剂量丝瓜组小鼠血清SOD分别为(337.00±29.73)U/mL和(349.00±9.99)U/mL,均高于高脂模型组和空白对照组,其中高剂量丝瓜组SOD活性明显高于高脂模型组(P<0.01)。结论:丝瓜多酚具有较强的抗氧化作用,丝瓜能明显降低高脂血症小鼠的体重、肝指数及血脂水平。
AIM： To observe the antioxidation and the serum lipid lowing effects of polyphenols in luffa cylin-drical on experimental hypedipidemia mice. METHODS： Acetone was used to extract polyphenols from luffa cylindrical. The content of polyphenols was determined by Folin - ciocalteau method. The inhibitory efficacy of the extracts from luffa cylindrical to the production of OH.free radical was measured. Kunming mice were used to establish the hyperlipidemia model by feeding high fat diet. The freeze - dried fresh luffa cylindricals was added to the treated animals, xuezikang was also used as a positive control. After feeding high - fat food for 14 days, serum were collected to measure the total choles- terol （TC） , triglyceride （TG） , low density lipoprotein cholesterol （LDL- C） and superoxidedismutase （SOD）. The liver tissue was also collected for determining malonaldehyde （MDA） content. RESULTS ： （1） Content of polyphenols in fresh luffa cylindrical was （0.3±0.1）g/kg. （2） The polyphenol extracts from fresh luffa cylindrical had a strong efficacy to inhibit the production of OH.free radicals. （3） The trend of body weight gain in groups LC1 （low dosage luffa cylindrical） and LC2 （high dosage luffa cylindrical） became slow down obviously, compared with that in group HPL （high -lipid model）. （4） The levels of TC （4.19±0.37） mmoL/L and LDL - C （2.77±0.79） mmoL/L in group LC1, TC （3.56±0.55） mmoL/L and LDL - C （2.34±0.41 ） mmoL/L in group LC2, TC （4.59±0.96） mmoL/L and LDL - C （ 3.25±0.67 ） mmoL/L in group PC （positive control） were all lower than those in group HPL（P 〈0. 01 ）, which the decrease in the levels of TC and LDL- C in group LC2 was the most obvious. （5） SOD levels in the serum of mice in groups LC1 and LC2 were （337.00±29.73 ） U/mL and （ 349.00±9.99 ） U/mL respectively, which were higher than those in groups HPL and blank control, therein the SOD level in group LC2 increased obviously（ P 〈 0. 01 ） compared with group HPL. The MDA levels in liver tissue in group LC2 was lower than those in control. CONCLUSION： Polyphenols in the luffa cylindrical are comparatively strong antioxidant. They effectively decrease the liver index and serum lipid in experimental mice.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2008年第5期873-877,共5页
Chinese Journal of Pathophysiology
基金
广东省中医药管理局资助项目(No.粤中医[2004]26号)
国家中医药管理局三级实验室开放课题基金资助项目(No.2005)
关键词
丝瓜
高脂血症
多酚
抗氧化
Luffa
Hyperlipidemia
Polyphenol
Antioxidation
