摘要
Objective： To observe the feasibility of gene silencing of MAT1 gene by small interference RNA in human pan- creatic cancer cells. Methods： BxPC-3 cells were transfected using chemically synthesized double stranded RNA formulated with liposome. Gene expression of MAT1 was assayed by RT-PCR and Western blot respectively. The cell proliferation as- say was carried out by counting alive cells after Trypan blue exclusion. The cells invasion ability was determined by Boyden chamber model. Results： The MAT1 mRNA and protein expression of BxPC-3 cells were significantly down-regulated by small interference RNA compared with the control groups. The expression of MAT1 mRNA was reduced by 55.2% and 64.3% in 24 h and 48 h respectively （P 〈 0.01）. The cell proliferation and invasion ability of BxPC-3 cell were significantly inhibited （P 〈 0.01）. Conclusion： The results suggest that gene silencing of MAT1 by siRNA can inhibit the cell proliferation and invasion of BxPC-3 cells, which may be a target in the gene therapy of human pancreatic cancer.
基金
a grant from the Guangdong Science and Technology Commission(No.04009381)
