摘要
目的建立测定复方青骨凝胶中大黄素含量的方法。方法采用RP-HPLC法，以Waters Xterra RP18（3．9mm×150mm，5．0μm）为色谱柱；甲醇-水-磷酸（体积比80：20：0．1）为流动相；检测波长：440nm；流速：1．0mL／min；柱温：30℃。结果大黄素的进样量在0．3524～1．7620μg范围内与峰面积呈良好的线性关系，r=0．9999；平均加样回收率为101．99％，RSD为2．79％（n=6）。结论本方法简便、快速、准确，可作为复方青骨凝胶中大黄素的含量测定方法。
Objective To establish a RP-HPLC method for determining the content of emodin in Fufang Qinggu gel. Method The sample was analyzed on a Waters Xterra RP1s （3.9 mm × 150 mm, 5.0 μm） column by using methanol-water-H3PO4 （80： 20：0.1 ） as mobile phase. The detection wavelength was at 440 nm and flow rate 1.0 mL · min^-1. Result The linear range of sinomenine was 0. 352 4- 1. 762 0 μg （r = 0.999 9）. The average recovery was 101.99% with RSD 2.79% （n = 6 ）. Conclusion This method is simple, rapid and accurate which could be used for determining the content of emodin in Fufang Qinggu gel.
出处
《广东药学院学报》
CAS
2008年第2期121-122,125,共3页
Academic Journal of Guangdong College of Pharmacy
