摘要
目的探讨不育患者精子活力与精浆及精子DNA完整性的关系。方法50例男性不育患者,按a+b级活力分为2组,a+b级精子百分率≥50%为Ⅰ组,<30%为Ⅱ组,Ⅰ组25例,Ⅱ组25例。各组精液分成两部分,一部分用于两组间精浆互换,另一部分用作各自的对照。精浆互换后37℃温育40min、90min,用计算机辅助精液分析系统(CASA)分析精浆互换前后及其对照组的a+b级精子百分率。同时检测这些患者的精浆α-葡糖苷酶、酸性磷酸酶和果糖含量,并用吖啶橙荧光染色检测随机抽取的40名患者精子核DNA的完整性。结果Ⅰ组与Ⅱ组精浆置换后,温育40min和90min时Ⅰ组的精浆置换组精子活力为27.5±18.7、25.8±17.1,比相应的对照组33.9±17.3、26.0±15.6降低;Ⅱ组的精浆置换组精子活力为16.7±11.6、14.4±10.5,比相应的对照组12.2±8.7、11.2±9.3升高;两组的精浆置换组与相应的对照组的精子活力均随着温育时间的延长而降低,但均无统计学意义。精浆果糖、酸性磷酸酶与精子活力间没有相关性,而精浆α-葡糖苷酶与Ⅰ组的精子活力呈正相关。精子活力与精子核DNA的完整性呈正相关性。结论精子活力主要与精子结构有关,精浆成分也是决定精子活力的一个因素。
Objective To investigate the relationship of sperm motility with seminal plasma and sperm DNA integrity in male patients with infertility. Methods A total of 54 infertility patients was divided into two groups according to sperm motility of grade （a ＋ b）. In Group 1 （n =25） the percentage of sperm with grade （a ＋ b） was ≥ 50%, while in Group 2 （n =29） the percentage of grade （a ＋ b） was 〈30%. The seminal plasma of both the 2 groups were divided into two parts, the first one was used to exchange between the 2 groups, and the rest was as control. Alter exchanging, the samples of both the 2 groups were incubated at 37℃ for 40 min and 90 min, then the seminal plasma with percentage of grade （a ＋ b） were analyzed with CASA system. Meanwhile, the concentration of alpha- glucosidase, acid phosphotase （ACP）, and fructose （Fru） were detected. The integrity of sperm DNA frora 40 random samples was determined with acridine orange fluorescence method. Results After exchange between the 2 groups, the percentage of seminal plasma with grade （ a ＋ b） in group 1 was lower than that in control whether they were incubated for 40 min or 90 rain. Meanwhile, after exchange, the percentage of seminal plasma with grade （ a ＋ b） in group 2 were higher than that in control. There was no statistically significant difference between the 2 groups. In both the two groups, the percentage of sperm with grade （ a ＋ b） decreased with incubation tirae. There was no correlation between the concentration of ACP and Fru in seminal plasma and sperm motility, but a positive correlation between sperm motility and alpha- glucosidase concentration, sperm motility and sperm DNA integrity in Group Ⅰ was found. Conclusion Sperm DNA integrity play important role for sperm motility, while the components in seminal plasma might be the factors that affects sperm motility.
出处
《临床检验杂志》
CAS
CSCD
北大核心
2008年第3期191-193,共3页
Chinese Journal of Clinical Laboratory Science
基金
江苏省自然科学基金(BK2006135)
全军"十一五"医药卫生科研基金(06H011)
关键词
精子活力
精浆
置换
精子DNA完整性
不育患者
sperm motility
seminal plasma
exchange
sperm DNA integrity
infertility patients
