摘要
目的了解使用无铅化汽油后汽车尾气的生殖毒性效应。方法30只SD大鼠等分为实验和对照组,根据染毒时间4、6和8周不等各分为3个亚组,对照组用空白吸收液灌胃,实验组用汽车尾气吸收液灌胃,每周5 d,每天1次,灌胃量0.1 ml/L。观察大鼠生殖毒性及睾丸组织微量元素的变化。结果尾气吸收液染毒大鼠体重变化不明显;睾丸、附睾的脏器系数低于同时相对照组(P<0.05),且随时间呈下降趋势;精子含量和存活率显著降低,而畸形率增高(P<0.05);睾丸中Zn、Mn代谢紊乱,出现Zn低、Mn高,有时间-效应关系。结论汽车尾气具有明显的生殖毒性,微量元素代谢紊乱对生殖腺的损害起了重要作用。
Objective To investigate the gonad toxicity of exhaust gas from cars using lead- free gasoline and the influences on trace elements in rats. Methods Thirty male SD rats were randomly divided into the experiment group （n= 15） and the control group （n= 15）. The rats in the experiment group were administrated by oral gavage with the absorbent solution containing car exhaust at a dose of 0.1 ml/L only once a day; and the rats in the control group were administrated by oral gavage with placebo absorbent solution. The changes in body weight, spermatogenesis and contents of trace elements were examined in testes after oral gavage for 4, 6 and 8 w. Results The ratios of gonads to body weight in the rats of experiment group were lower than those in the control group （P〈 0.05） and fewer alive sperms and more malformed or dead sperms were found in the epididymis of rats exposed to car exhaust for 6 and 8. The content of Zn in the testis was significantly decreased but that of Mn significantly increased in the rats exposed to car exhaust for longer period, and a time - effect relationship was demonstrated. Conclusions Exhaust gas from cars using lead- free gasoline significantly induces toxicity in male rat gonads,and the imbalance of Zn and Mn might be contributive to the damage of testis.
出处
《实用预防医学》
CAS
2008年第2期306-307,共2页
Practical Preventive Medicine
基金
湖南省教育厅科研项目(编号:4-02-JY-02C393)
