摘要
目的探讨IL-18与T2DM发病的关系。方法72只雄性SD大鼠随机分成NC、NCS、HF和HFS组．第8周末HFS与NCS组按25mg／kg腹腔注射无菌链脲佐菌素（STZ）溶液造模，NC与HF组注射相应无菌柠檬酸钠溶液比较，2周后行OGTT筛选成模大鼠，分别于14和20周末将四组大鼠各处死一半，取血清样本，用ELISA法检测IL-18、IL-6、TNF-α水平。结果HFS组大鼠达到成模标准，其他组未成模。第14和20周末，HFS组IL-18、IL-6、TNF-α水平均显著高于相同周数NC组（P〈0．01）。但20周末HFS组IL-18、IL-6、TNF—α水平与第14周末比较，差异无统计学意义（P〉0．05）。Pearson直线相关分析显示，第14和20周末，HFS组IL-18均分别与FBG、IL-6、TNF-α呈正相关（r=0．90，P〈0．01或r≥0．73，P〈0．05），与ISI呈负相关（r=-0．86，P〈0．01）。结论本实验成功建立了符合人类临床特点的T2DM大鼠模型，成模率81．8％；IL-18等炎症因子与T2DM发病密切相关．慢性炎症在他DM发病过程中起重要作用。
Objective To investigate the relationship between interleukin-18 （IL-18） and the pathogenesis of type 2 diabetes mellitus. Methods 72 healthy Spragne-Dawlay male rats were randomly divided into four groups, NC group, NCS group, HF group and HFS group. At the end of the 8th week, NCS group and HFS group were injected with STZ （25mg/kg） into abdominal cavity. At the end of the 10th week, diabetic rats were screened by oral glucose tolerance test （OGTF）. The blood sample was collected when the rats were killed at the end of the 14th and 20th week. The levels of serum IL-18, IL-6 and tumor necrosis factor-α （TNF-α） were assayed with ELISA. Results Most rats in HFS group were achieved the diagnostic standard of diabetic rat, and their insulin sensitivity index （ISI） were decreased. At the end of the 14th week and 20th week, the levels of serum IL-18, IL-6 and TNF-α in HFS group were significantly higher than those in NC group （ P 〈0.01 ）. In HFS group, the levels of serum IL-18, IL-6 and TNF-α at the end of the 20th week were higher than those at the end of the 14th week, but it had no statistic significance （ P 〉0. 05）. Pearson linear correlation analysis showed that the level of serum IL-18 in HFS group was positive correlated with FBG, IL-6, and TNF-α （ r =0. 90, P 〈0. 01 or r ≥0. 73, P 〈0.05）, and nega- tive correlated with ISI （ r = -0. 86, P 〈 0. 01 ）. Conclusions Our results show that IL-18 is related with the pathogenesis of type 2 diabetes mellitus and chronic inflammation plays an important role in the development of type 2 diabetes mellitus.
出处
《中国医师杂志》
CAS
2008年第5期580-583,共4页
Journal of Chinese Physician
基金
2005年广东省医学科学技术研究基金（A2005480）
