摘要
为研究常春油麻藤Mucuna sempervirens的抗旱机制,利用聚乙二醇(PEG 6000)模拟50 g.L-1(轻度干旱胁迫)和200 g.L-1(重度干旱胁迫)2种质量浓度的干旱胁迫环境,分别处理1,3和5 d后,测定常春油麻藤在干旱胁迫下的生理指标,并以中华常春藤Hedera nepalensis var.sinensis和扶芳藤Euonymus fortunei为对比材料,比较分析常春油麻藤的抗旱性。研究表明,随着PEG 6000质量浓度和处理时间的增加,3种藤本的叶片含水量和失水速率均呈下降趋势,而叶片水分饱和亏缺、细胞膜透性和游离脯氨酸指标数均显著上升,超氧化物岐化酶活性变化呈先上升后下降趋势。重度胁迫下,常春油麻藤的游离脯氨酸质量分数显著高于扶芳藤和常春藤,而细胞膜透性却上升幅度不大。3种被测藤本中抗旱性排序为:扶芳藤>常春油麻藤>中华常春藤。常春油麻藤主要通过提高体内渗透调节物质的积累和保护酶活性来抵抗干旱胁迫,保护细胞膜结构,提高抗旱性。
To understand drought tolerance in Mucuna sempervirens, physiological and morphological expression studies were done on three species of liana （Hedera nepalensis var. sinensis, and Euonymus fortunei） with polyethylene glycol （PEG） 6000 concentrations of 50 g·L^-1 and 200g·L^-1 for 0 （ the control）, 1, 3, and 5 d. All of the samples were two-year-old seedlings. Results showed that leaf water content and the rate of dehydration decreased, but water saturation deficit, electrolyte leakage, and free proline increased significantly （P 〈0.05）. Superoxide dismutase （SOD） increased at first, but then decreased. Under strong drought resistance, free proline in contents for M. sempervirens was significantly （P 〈0. 05） more than those of 1t. nepalensis var. sinensis and E. fortunei. Synthesize all of the indexes, drought resistance of the three lianas was E. fortunei 〉 M. sempervirens 〉 H. nepalensis var. sinensis. During the stressed period, proline content and SOD activity of M. sempervirens increased to resist drought and protect the cell membranes. [ Ch, 8 fig. 2 tab. 15 ref. ]
出处
《浙江林学院学报》
CAS
CSCD
北大核心
2008年第3期314-318,共5页
Journal of Zhejiang Forestry College
基金
浙江省科学技术攻关项目(2004C32068)
浙江省林业厅资助项目(2002A18)
浙江省临安市科技局资助项目(2002)
关键词
植物学
抗旱性
聚乙二醇(PEG6000)
生理指标
常春油麻藤
中华常春藤
扶芳藤
botany
drought resistance
polyethylene glycol （ PEG 6000）
physiological index
Mucuna sempervirens
Hedera nepalensis var. sinensis
Euonymus fortunei
