摘要
目的:研究冬凌草甲素(oridonin,ORI)诱导人食管癌SHEE细胞凋亡的效应及其机制。方法:用不同浓度ORI分别作用人食管癌SHEE细胞株不同时间,噻唑蓝比色法(MTT)检测细胞生长抑制率;透射电镜观察细胞超微结构及改变;原位末端标记/碘化吡啶(TUNEL/PI)双染流式细胞术检测细胞凋亡率和细胞周期。结果:ORI对SHEE细胞的生长有显著的抑制作用,64μg/ml的ORI作用24h、48h及72h对SHEE细胞生长的抑制率分别为78.7%、89.5%及91.7%;32μg/ml的ORI作用2h后,电镜下可见线粒体在细胞核出现变化之前已发生形状改变,作用8h后,线粒体内部结构消失,细胞核呈细胞凋亡的典型改变;作用24h后,流式细胞仪检测凋亡率为47.7%,细胞周期图像显示明显的凋亡峰。结论:ORI可诱导SHEE细胞凋亡,其机制可能与线粒体凋亡途径有关。
Objective：To study the effect and mechanism of the apoptosis of human esophageal carcinoma cell line SHEE induced by oridonin（ORI）. Methods： After being treated by ORI in different density during different time, we detected the rates of the growth inhibition of human esophageal carcinoma cell line SHEE by Methyl Thiazolyl Tet- razolium（MTF） assay; Observe the change of the ultramicrostructure of the cells under transmission electron microscope; Using double - dye assay of in situ end - labeling/iodinate pyridina（TUNEL/PI）, the rates of the apoptotic cells and the phrase distribution of cell cycle were detected by flow cytometry. Results： ORI could inhibit the cell growth of SHEE conspicuously. The inhibition ratio reached 78.7% with ORI in a density of 64μg/ml after 24 hours. If the time lasted to 48 hours, the inhibition ratio would be 89.5%, the rate was 91.7% after 72 hours; Having been treated by ORI in a density of 32μg/ml in 2 hours, the morphous of mitochondrium changed ahead of cell nucleus under transmission electron microscope. After 8 hours, the internal structure of mitochondrium disappeared, and the typical apoptotic morphous of cell nucleus could be seen ; When the time of the treatment prolong to 24 hours, the apoptosis ratio was 47.7% according to flow cytometry. An evident apoptotic peak displayed on the phrase distribution of cell cycle. Conclusion： ORI could induce apoptosis on the cell line SHEE by the mechanism of mitochondrium apoptotic pathway probably.
出处
《现代肿瘤医学》
CAS
2008年第6期907-910,共4页
Journal of Modern Oncology
基金
广东省科技计划资助项目(编号:2006B35630009)
关键词
冬凌草甲素
人食管癌SHEE细胞
凋亡
线粒体
oridonin（ORI）
human esophageal carcinoma cell line SHEE
apoptosis
mitochondrium
