摘要
为了克隆小麦干旱应答基因,构建了干旱诱导的小麦cDNA文库,并从文库中分离了一个DREB类转录因子基因TaDREB6。序列分析表明,TaDREB6具有一个837 bp的开放阅读框和242 bp的3'非编码区,推测的氨基酸序列中含有一个高度保守的AP2/EREBP结构域。采用该基因特异引物PCR技术对一套中国春缺体-四体材料进行扩增,将TaDREB6定位于3A染色体上,这是首次将一个小麦DREB基因定位在特定的染色体上。RT-PCR分析表明,TaDREB6基因受干旱胁迫诱导表达;亚细胞定位结果表明,TaDREB6-hGFP融合蛋白定位于细胞核中。上述结果说明,小麦TaDREB6基因编码的蛋白可能在细胞核内对干旱胁迫应答反应起调控作用。
In search for genes response to drought stress in wheat （Triticum aestivum L. ）, a cDNA library was constructed from drought-treated wheat seedlings, and a DREB-like transcription factor gene, TaDREB6 was isolated. TaDREB6 had an open reading frame of 837 bp with a 3＇-UTR of 242 bp, with a highly conserved AP2/EREBP domain in the encoded putative protein. Using a nullisomic-tetrasomic series of T. aestivum L. cv. Chinese Spring, the TaDREB6 gene was located on chromo-some 3A. The result of RT-RCR indicated that TaDREB6 gene was obviously induced by drought stress, suggesting an important role of TaDREB6 in response to drought stress in wheat. Subcellular localization assay indicated the TaDREB6-hGFP fusion protein was targeted in the nucleus. Above results suggested that TaDREB6 transcription factor might be involved in drought stress response and functioned in the nucleus.
出处
《麦类作物学报》
CAS
CSCD
北大核心
2008年第3期357-363,共7页
Journal of Triticeae Crops
基金
国家高技术研究发展计划(“863”计划)项目(2007AA10Z130)
国家自然科学基金项目(30700504)
北京市科技计划项目小麦转基因育种(Z07070501770702)
关键词
小麦
干旱
DREB
基因克隆
Triticum aestivum L.
Drought
DREB
Gene clone
