摘要
目的探讨重症急性胰腺炎(SAP)大鼠肝组织高迁移率族蛋白B1(HMGB-1)表达及其与肝功能损害的关系。方法逆行胰胆管注射5%牛磺胆酸钠制作Wistar大鼠SAP模型。随机分成3组:对照组、SAP组和二硫代氨基甲酸吡咯烷处理组(PDTC组)。采用RT-PCR和免疫组织化学法检测肝组织HMGB-1mRNA和蛋白的表达水平。同时检测血浆天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)水平变化。结果大鼠肝组织HMGB-1mRNA表达SAP组较对照组明显增加,PDTC组较SAP组显著减少(P<0.05)。SAP组HMGB-1蛋白在肝细胞和枯否细胞表达较强,而在对照组表达较弱,PDTC组HMGB-1蛋白表达较SAP组显著减弱。同时,SAP组大鼠血浆AST、ALT水平较对照组升高,而PDTC组较SAP组明显降低(P<0.05)。结论SAP大鼠肝组织HMGB-1表达升高参与了肝损伤的病理生理过程,PDTC可通过抑制肝组织HMGB-1表达进而改善肝功能。
Objective To explore the expression of hepatic high mobility group box-1 protein （HMGB-1） and its relationship with hepatic function injury. Methods SAP model was established by retrograde injection of 5% sodium taurocholate into pancreatic duct. Animals were divided randomly into 3 groups：Control group,SAP groups and PDTC group. Expression of HMGB-1 in liver was measured with RT-PCR and immunohistochemistry,and plasma AST and ALT levels were also measured. Results HMGB-1 mRNA expression in Liver increased in SAP group,while markedly decreased in PDTC group compared with that in SAP group （P 〈 0.05）. HMGB-1 protein expression in hepatocytes and Kupffer＇s ceils in SAP group was significantly up-regulated compared with that of normal control group. Plasma AST and ALT levels were significantly higher in SAP group than that in Control group （P 〈 0.05 ）. The positive rate of HMGB-1 expression in hepatocytes and Kupffer＇s ceUs and the levels of plasma AST and ALT were higher in SAP group than those in control group, but were significantly reduced in PDTC treatment group （P 〈 0.05 ）. Conclusion PDTC can down-regulate hepatic HMGB-1 expression and improve liver function in SAP rats, Excessive HMGB-1 formation in liver might be involved in the pathogenesis of acute hepatic injury of SAP.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2008年第3期343-345,共3页
Journal of China Medical University
