摘要
采用正交试验设计对麸皮中燕麦生物碱(AV)的提取条件进行优化,提取物经大孔吸附树脂纯化,并利用活性氧和DPPH自由基的清除率研究其抗氧化活性。结果表明:最佳提取参数为温度60℃,提取时间2h,溶剂为乙醇/水/冰醋酸(80/19.9/0.1),料液比1︰8,此条件下得率为5.29%,经树脂纯化后粗提物中生物碱纯度达19.2%,且纯化后的燕麦生物碱表现出较强的清除OH.,O2-.和有机自由基DPPH的体外抗氧化活性,其清除能力分别是α-生育酚的79.4%、82.2%和78.0%。
The technology for extraction avenanthramides from oat bran was optimized by Orthogonal design, and purified by macroporous resin; Meanwhile, the vitro antioxidant activity were studied. Results show that the optimal parameters for extraction are ethanol / water / acetic acid 80/19.9/0.1, temperature 60℃, solid-liquid ratio of 1 ： 8, the extraction time is 2 h. Under such conditions, the yield of avenanthramides is 5.29%, the content of avenanthramides can be increased up to 19.2% after being purified by AB-8 macroporous resin. It also show that the purified avenanthramides extract has strong capacity of scavenging OH·, O2-· and DPPH free radical, the scavenging capacity are 79.4%, 82.2% and 78.0% as large as that of ct-tocopherol respectively.
出处
《农业工程学报》
EI
CAS
CSCD
北大核心
2008年第5期265-269,共5页
Transactions of the Chinese Society of Agricultural Engineering
基金
国家公益研究专项基金(2005DIA4J019)
关键词
燕麦麸皮
生物碱
提取
抗氧化活性
oat bran
avenanthramides
extraction
antioxidant activity
