摘要
目的:建立一个基于连接酶检测反应(LDR)的基因多态性检测系统,探讨降钙素基因相关肽(calcitoningene-relatedpeptide,CGRP)和白介素-1A(Interleukin-1A,IL-1A)基因多态性与中国汉族人群重度慢性牙周炎遗传易感性的关系。方法:收集100例重度慢性牙周炎病人和118例健康对照组的颊黏膜拭子并抽提DNA,应用PCR-LDR方法检测CGRP1210、CGRP4218、CGRP+5247以及IL-1A-889基因型,并使用软件SHEsis进行单体型分析,进行各组间基因型分布和等位基因频率的χ2检验。结果:重度慢性牙周炎病人组CGRP1210、CGRP+5247以及IL-1A-889阳性基因型均显著高于健康对照组(P<0.05),CGRP4218的基因型、等位基因频率在各组间的分布无显著性差异(P>0.05)。结论:建立了一个基于连接酶检测反应的基因多态性并行检测系统,IL-1、CGRP基因多态性与重度慢性牙周炎有相关关系,IL-1A-889、CGRP1210和CGRP+5247阳性基因型可能是重度慢性牙周炎易感性的遗传标志,同时提示IL-1、CGRP基因型在不同地域、不同人种中的分布可能是不相同的。
To establisha parallel typing system based on ligase detection reaction （LDR）, and to study the correlation between CGRP and IL - 1A gene polymorphism and the susceptibility to severe chronic periodontitis in Chinese Han nationality. METHODS： Buccal swabs were collected from 100 adult patients with severe chronic periodontitis and 118 healthy adults. DNA was extracted from each sample. PCR -LDR technique was used to identify CGRPI210, CGRP ＋ 5247, CGRP4218 and IL - 1A -889 genotype. The data were analyzed by χ^2 test using SHEsis. RESULTS： The results showed a significant difference in the gene type of IL - 1A -889, CGRPI210 and CGRP ＋ 5247 between patients with severe chronic periodontitis and periodontally healthy controls. CONCLUSION： The parallel typing system based on ligase detection reaction was established for gene polymorphism research. There is a possible role of IL - IA and CGRP gene polymorphisms in the susceptibility to severe chronic periodontitis for some patients.
出处
《牙体牙髓牙周病学杂志》
CAS
2008年第5期241-246,共6页
Chinese Journal of Conservative Dentistry
基金
上海市自然科学基金资助课题(06ZR14123)
关键词
连接酶检测反应
降钙素基因相关肽
白细胞介素-1
基因多态性
重度慢性牙周炎
ligase detection reaction
calcitonin gene - relatedpeptide（CGRP）
interleukin - 1 （ IL - 1 ）
gene polymorphisms
severe chronic periodontitis
