摘要
目的建立测定人体内血浆中奥美沙坦浓度的HPLC-MS方法。方法采用三氟乙酸沉淀法处理血浆,HyPurityC18柱(150mm2.1mm,5μm),柱温40℃,流动相为水-甲醇-乙腈(14:60:26),流速为0.22mL·min-1,质谱条件采用正离子检测的电喷雾电离方式,喷雾电压4.5kV,正离子模式,用于定量分析的离子(m/z)分别为447(奥美沙坦),376(内标,加替沙星)。结果奥美沙坦检测下限为25μg·L-1,线性范围25～3200μg·L-1(r=0.9998),日内和日间RSD均低于15%。结论该方法灵敏度高、快速、简便、无杂质干扰,适合于奥美沙坦的药代动力学及生物等效性研究。
Objective To establish a simple and rapid HPLC-MS method for determining the contents of olmesartan in human plasma. Methods Plasma were precipitated with trifluoroacetic acid, then analyzed on an HyPurity C18 column （150 mm 2.1 mm, 5 μm）. Samples at 40 ℃. The mobile phase consisted of water-methanolacetonitrile（14：60：26） with a flow rate of 0.22 ml/min. Results The lower limit of qualification was 25 μg/L. The calibration curve was linear over the range of 25-3200 μg/L （r=0.9998）, with the intra-day and inter-day RSD less than 15%. Conclusion The method is sensitive, rapid and suitable for the study ofpharmacokinetics and bioavailability of olmesartan.
出处
《南方医科大学学报》
CAS
CSCD
北大核心
2008年第6期1104-1105,共2页
Journal of Southern Medical University
