摘要
目的探讨亚甲基四氢叶酸还原酶（MTHFR）基因C677T多态性与福建汉族人群高血压病（EH）血瘀证易感性的关系。方法收集高血压病血瘀证患者239例,高血压病非血瘀证患者203例,血压正常对照患者195例,抽外周静脉血提取DNA,采用聚合酶链反应-限制性内切酶片段长度多态性（PCR-RFLP）检测MTHFR基因C677T多态性类型。结果三组间CC、CT和TT基因型构成比分布有显著性差异（χ^2=14.268,P=0.006）;经进一步卡方分割检验显示高血压病血瘀证组CT＋TT基因型频率（45.61%）高于高血压病非血瘀证组（35.96%）和对照组（29.23%）,差异显著（χ^2=10.672,P=0.001）,调整相关混杂因素后,CT/TT基因型人群患高血压病血瘀证的相对危险度是CC基因型的1.943倍（95%,CI：1.271-2.970,P=0.002）。结论MTHFR基因CT/TT类型可能是汉族人群高血压病血瘀证发病的易感危险因素之一。
Objective To explore the relationship between the C677T single nucleotide polymorphism of 5,10-methylenetetrahydrofolate reductase（MTHFR） and blood stasis syndrome（BSS） of essential hypertension（EH） in Han people of Fujian.Methods The MTHFR C677T genotypes in 239 patients of EH of BSS type（BSS-EH group）,203 patients of EH of non-BSS type（non-BSS-EH group） and 195 healthy subjects（control group） were determined by polymerase chain reaction-restriction fragment length polymorphism（PCR-RFLP） assay.Results The constituent ratio of MTHFR CC、CT and TT genotype was significantly different among three groups（χ^2 =14.268,P=0.006）.Partitions of χ^2 method showed that the frequencies of CT/TT genotype in patients with BSS-EH（45.61%） were higher than that of non-BSS-EH group（35.96%） and control group（29.23%）（χ^2=10.672,P=0.001）,and multinomial logistic-regression indicated that CT/TT genotype was an independent risk factor for BSS-EH with the reference category of control.The odds ratio（OR） of the CT/TT genotype for BSS-EH was 1.943（95% confidence interval： 1.271 to 2.970,P=0.002） after adjusting for age,gender,fasting plasma glucose,creatinine,body mass index（BMI）, uric acid and blood fat.Conclusion The MTHFR CT/TT genotype maybe one of the risk factors associated with the susceptivity to BSS-EH in Chinese Han people.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2008年第5期351-354,共4页
Journal of Beijing University of Traditional Chinese Medicine
基金
福建省科技计划资助项目(No.2005D038)
福建省自然科学基金重点资助项目(No.C0420003)
关键词
高血压
血瘀证
亚甲基四氢叶酸还原酶
基因
hypertension
blood stasis
methylenetetrahydrofolate reductase
gene
