摘要
在摇瓶中考察了重组Pichia pastoris发酵的诱导剂量,L-甲硫氨酸,以及pH对腺苷甲硫氨酸产量的影响。放大到3.7 L发酵罐和30 L发酵罐后,研究了重组细胞的发酵过程变化,对S-腺苷甲硫氨酸初步纯化。摇瓶中优化后的发酵条件是:每天添加1%甲醇诱导,L-甲硫氨酸为50mmol/L,培养基pH 5.0。培养144 h后SAM产量达到2.32 g/L。3.7 L发酵罐中发酵251 h后细胞浓度为120 g/L,SAM总量为15.18 g。放大到30 L发酵罐中,发酵225.5 h后细胞浓度约为120 g/L,SAM总量为145.05 g。纯化后SAM的纯度为93.5%,回收率为84.5%。
The fermentation process of S-adenosylmethionine biosynthesizing using recombinant Pichia pastoris in flasks was optimized and scaled-up in 3.7 L and 30 L bioreactor, respectively. The yield of SAM was 2.32 g/L at pH 5.0 with 50 mmol/L L-methionine and 1% methanol added per day in flask. In 3.7 L bioreactor, 120 g/L dry cell weight and 15.18 g SAM were obtained after 251 h cultivation. Dry cell weight and total yield of SAM were 120 g/L and 145.06 g respectively in 30L bioreactor after 225.5 h cultivation. SAM was purified to 93.5 % content with a recovery of 84.5 %.
出处
《工业微生物》
CAS
CSCD
北大核心
2008年第3期6-11,共6页
Industrial Microbiology
基金
上海华谊(集团)公司-华东理工大学先进化学与化工技术研究中心探索性研究项目资助
