摘要
采用热水提取、酒精沉淀、Sevag法去蛋白、有机溶剂脱水的方法提取库拉索芦荟花粗多糖,DEAE Sephadex A-25阴离子交换层析法从粗多糖中分离出中性多糖、弱酸性多糖和强酸性多糖。其中弱酸性多糖含量最高,约占多糖的87%,这不同于库拉索凝胶多糖,凝胶多糖主要由中性多糖组成,占总多糖的95%以上。进一步采用Sepharose4B凝胶过滤层析分级分离,测出单一多糖的分子量,并用气相色谱法测定出多糖的中性单糖组成。
Crude flower polysaccharides from Aloe vera were isolated by a series of sequential steps including precipitation with ethanol, removal of protein by Sevag method, dehydration by several organic solvents. Polysaccharides from Aloe vera flower obtained after DEAE Sephadex A-25 anion-exchange chromatography consist of neutral polysaccharide,weakly acidic and strongly acidic polysaccharides, largely composed of weakly acidic polysaccharides （87%）,which was quite different from Aloe vera gel polysaccharides. For Aloe vera gel polysaccharides, neutral polysaccharides accounted for more than 95% of the total polysaccharides. The polysaccharides were further purified and their molecular weights were measured by Sepharose 4B gel permeation chromatography. Neutral sugar analysis was performed by gas liquid chromatography （GLC）.
出处
《食品工业科技》
CAS
CSCD
北大核心
2008年第6期79-81,共3页
Science and Technology of Food Industry
基金
烟台大学青年基金(HY05Z2)
国家“十五”-863课题(2001AA627040)
关键词
库拉索芦荟花
多糖
分离
层析
Aloe vera flower
polysaccharides
isolation
chromatography
