摘要
目的探讨红花对兔肺缺血-再灌注损伤时细胞间黏附分子-1（ICAM-1）表达的影响及其机制。方法复制在体兔单肺原位缺血．再灌注损伤模型。日本大耳兔30只，由温州医学院实验动物中心提供，随机分为3组（每组10只）：假手术对照组（S组），缺血-再灌注组（I／R组）和缺血-再灌注＋红花注射液组（SI组）。SI组分别在缺血前20min和再灌注即刻静脉注射红花注射液（2．0ml／kg），实验结束时，白颈动脉抽血检测丙二醛（MDA）含量、超氧化物歧化酶（SOD）和黄嘌呤氧化酶（XO）活力；取肺组织测湿干重比（W／D），髓过氧化物酶（MPO）活力；电镜观察肺组织超微结构改变；免疫组化法检测肺组织ICAM-1蛋白表达。统计学处理采用单因素方差分析。结果I／R组血清MDA、XO均显著高于S组（P〈0．01），SOD明显低于S组（P〈0．01）；I／R组和SI组的W／D与MPO均高于S组（P〈0．05或P〈0．01），但SI组显著低于I／R组（P〈0．01）；I／R组肺组织的超微结构损伤严重，SI组损伤程度明显较轻；免疫组化发现I／R组肺组织ICAM-1（2．94±0．48）蛋白表达显著高于SI（1．75±0．62）组（P〈0．01）。结论红花可通过下调ICAM-1蛋白的表达，抑制中性粒细胞聚集，降低氧自由基水平，减轻脂质过氧化反应，从而减轻肺缺血-再灌注损伤。
Objective To investigate the influence of injection carthamus tinctorius D. （IC） on the expression of intercellular adhesion molecule-1 （ICAM-1） during the ischemia-reperfusion injury of lung （LIRI） in rabbits and its potential mechanism. Method Single lung ischemia-reperfusion animal model was induced in rabbits. A total of 30 Japanese white rabbits were randomly divided into sham-operation group （S group, n = 10）,ischemiareperfusion group （I/R group, n = 10） and ischemia-reperfusion plus IC group （IC group, n = 10）.The rabbits of IC group received IC 2.0 ml/kg injected intravenously just at 20 min before ligation of artery involved and the same dose of IC instantly at the initiation of reperfusion. Malondialdehyde （MDA）, superoxide dismutase （SOD） and xanthine oxidase（XO） in serum were measured. The lung tissue was sampled and assayed wet/dry weight ratio （W/D）, contents of myeloperoxidase （MPO） at the end of the experiment, and ultrastructure changes were observed under electron microscope. The expression of ICAM-1 was measured by using immunohistochemistry（ IHC）. snd one-way ANOVA was used for statistical analysis. Results In I/R group, serum XO and MDA increased and SOD decreased,whereas the same pattern of changes but less magnitude happened in IC group （P 〈 0.01）. The values of W/D and MPO were much higher in I/R group,but lower in IC group. Under elan microscope,the ultrastructure of lung tissue showed pathological changes in the rabbits of I/R group, and these changes were greatly attenuated in the rabbits of IC group. The IHC showed that ICAM - 1 in lung tissue of I / R group was （ 2 . 9 4 ±0.48） which was significantly higher than that of IC group（ 1.75 ± 0.62） （ P 〈 0.01）. Conclusions Injection Carthamus tinctorius D. may meliorate the ischemia-reperfusion injury of lung by way of suppressing the expression of ICAM- 1, inhibiting neutrophil aggregation, lowering oxygen free radical level and decreasing lipid peroxidation.
出处
《中华急诊医学杂志》
CAS
CSCD
2008年第6期610-613,共4页
Chinese Journal of Emergency Medicine
基金
浙江省卫生厅科研基金资助项目（SWS00021）
关键词
红花注射液
肺
缺血再灌注损伤
超微结构
细胞间黏附分子-1
Safflor injection
Lung
Ischemia-reperfusion injury
Ultrastructure
Intercellular adhesion molecule- 1
