摘要
目的:从肿瘤坏死因子-α(TNF-α)、黏附分子(ICAM-1、VCAM-1)表达变化揭示脑脉通对老龄大鼠脑缺血再灌注损伤保护机制及其量效关系。方法:采用大脑中动脉栓塞(middlecerebralarteryocclusion,MCAO)法复制脑缺血(缺血3h再灌注12d)动物模型。观察脑脉通(大、中、小剂量)对脑缺血再灌注老龄大鼠神经症状积分、脑组织含水量、病理变化、TNF-α、VCAM-1、ICAM-1及ICAM-1mRNA表达的影响。结果:模型组大鼠脑组织含水量、神经症状积分、TNF-α、VCAM-1、ICAM-1及ICAM-1mRNA表达均高于假手术组;脑脉通组(大、中、小剂量)、尼莫地平组脑组织含水量、神经症状积分、TNF-α、VCAM-1、ICAM-1及ICAM-1mRNA表达均低于模型组;脑脉通中剂量组神经症状积分和VCAM-1、ICAM-1及ICAM-1mRNA的表达低于尼莫地平组;脑脉通中剂量组各项指标均低于脑脉通小剂量组。结论:脑脉通拮抗脑缺血再灌注损伤机制与抑制TNF-α、VCAM-1、ICAM-1等表达有关,且以脑脉通中剂量组效果最全面。
Objective： To study the protective effects and dosage-effect relationship of Naomaitong on focal cerebral ischemia reperfusion in aged rats.Methods ： Focal cerebral ischemia model （ ischemia 3h and I/R12d）was duplicated with MCAO. The changes of the nervous dysfunction score ,the water content of cerebral constitution and the expression of TNF- α ,VCAM- 1,ICAM-1 and its mRNA were observed. Results ：The nervous dysfunction score ,the water content of cerebral constitution, the expression of TNF-α ,VCAM-1,ICAM-1 and its mRNA in aged model group were higher than those of the sham-operated group;but all of these were decreased in three Naomaitong groups and Nimodipine group compared with that of aged model group;the nervous dysfunction score and the expression of VCAM-1,ICAM-1 mRNA in Naomaitong group（0.9g·kg^-1） were lighter than that of the Nimodipine group;the nervous dysfunction score,the water content of cerebral constitution, the expression of TNF- α ,VCAM-1,ICAM-1 and its mRNA in Naomaitong group（0.9g·g ^-1· d^-1） were higher than that of Naomaitong group（0.45g·kg^-1·d^-1）. Conclusion ：Naomaitong could protect brain cell from damage after focal cerebral ischemia reperfusion injury by inhibiting the expression of TNF- α ,VCAM-1,ICAM-1, with the middle dose of Naomaitong being more effective.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2008年第7期583-586,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然科学基金资助项目(No.30371812)
河南省高校创新人才基金项目(No.2001-10)
河南省杰出青年科学基金项目(No.0612000700)
