摘要
目的:探讨宫颈病变局部白细胞介素(IL)-2、IL-4的表达与宫颈人乳头状瘤病毒(HPV)感染的关系。方法:收集宫颈活检组织92例,其中宫颈浸润性鳞状细胞癌(鳞癌)组织38例,宫颈上皮内瘤样病变(CIN)组织22例,宫颈正常或炎症组织32例。采用原位杂交法检测HPV16/18DNA、IL-2mRNA、IL-4mRNA在样本中的表达,按HPV16/18表达阳性或阴性对IL-2、IL-4在样本中的表达进行分层分析。结果:IL-2、IL-4阳性表达率在HPV16/18阴性表达的各组间其差异均无统计学意义(P>0.05)。在HPV16/18阳性表达的正常或炎症组、CIN组、鳞癌组中IL-2阳性表达率分别为0.0%、50.0%、5.9%。IL-4阳性表达率分别为0.0%、75.0%、88.2%,其差异均有统计学意义(P<0.05或P<0.01)。结论:随着病变严重程度的增加,感染HPV的宫颈组织中IL-2表达先增加后减少,IL-4表达增加。
Objective： To explore the relationship between the expression in the situ of IL-2,IL-4 and the infection of HPV in the cervix lesions. Methods： Ninety-two biopsy cervical tissues which included 38 examples of cervical invasive squamous cell carcinoma,22 cases of CIN,and 32 of normal or inflammation cervical tissue were collected. In situ hybridization was used to detect the expression of HPV16/18 DNA,IL-2 mRNA and IL-4 mRNA in the examples. Stratification analysis was carried out to analyze the expression of IL-2 and IL-4 according to the expression of HPV. Results： There was no significant difference in the positive expression rates of IL-2 and IL-4 in the normal or inflammation cervical tissue,CIN and cervical cancer with HPV negative expression （P 〉 0.05）. The positive expression rates of IL-2 were 0.0%, 50.0% and 5.9% respectively. The positive expression rates of IL-4 were 0.0%, 75.0% and 88.2% respectively, and there was a significant difference （P 〈 0.05 ）. Conclusion： In the cervical tissues with HPV infection, the expression of IL-2 increases firstly and then reduces with the increase of cervical lesions.
出处
《天津医药》
CAS
北大核心
2008年第6期407-409,I0005,共4页
Tianjin Medical Journal
基金
河南省医学科技创新人才工程资助项目(项目编号:200303056)
