摘要
【目的】菌株Lj20是从辣椒植株根部分离得到的一株有抗真菌活性的植物内生放线菌。为了进一步开发利用这一放线菌,对其进行了鉴定及抗菌活性物质的研究。【方法】根据Lj20的形态特征、培养特征、生理生化特征、细胞壁组分和16S rDNA序列对其进行鉴定。结合GC-MS分析,合成了代谢产物中所含的抗真菌活性物质,并用菌丝生长抑制法测定其生物活性。【结果】Lj20菌株属于链霉菌属,与娄彻氏链霉菌(Streptomyces rochei)极为相似。代谢产物中含有2,6-二叔丁基对甲酚和3,5-二叔丁基-4-羟基-苯甲醚。两种化合物对番茄灰霉病菌的EC50值分别为237.04mg/L和186.48mg/L。【结论】菌株Lj20鉴定为娄彻氏链霉菌(Streptomyces rochei)。2,6-二叔丁基对甲酚和3,5-二叔丁基-4-羟基-苯甲醚对番茄灰霉病菌都有较强的抑制作用。
[Objective] Endophytic actinomycete Lj20 with antifungal activity was isolated from the roots of capsicum plants. We identified Lj20 and synthesized its antifungal substances. [Methods] Morphological, biological and biochemical characteristics, chemotaxonomy analysis and 16S rDNA sequences were used to identify Lj20, According to GC-MS extrapolation result, one of antifungal substances in the metabolites of Lj20 was chemically synthesized. The bioactivities were determined by mycelium growth inhibition method. [Results] Lj20 belonged to Streptomyces sp. and was similar to Streptomyces rochei. The metabolites contained butylated hydroxytoluene and 3, 5-di-tert-butyl-4-hydroxybenzyl methyl ether. The median effective concentration （EC50） of these two compounds to Botrytis cinerea Pers. were 237.04 mg/L of the water and 186.48 mg/L of the water, respectively. [Conclusion] Lj20 was classified as Streptomyces rochei. Butylated hvdroxvtoluene and 3, 5-di-tert-butyl-4-hydroxybenzyl methyl ether had significant inhibition to the pathogen.
出处
《微生物学报》
CAS
CSCD
北大核心
2008年第7期900-904,共5页
Acta Microbiologica Sinica
基金
山西省留学基金(200441)
山西省科技攻关项目(051045
2007031039)~~
关键词
内生放线菌Lj20
鉴定
抗真菌物质
合成
endophytic actinomycete Lj20
identification
antifungal substance
synthesis
