摘要
目的观察甲基叔丁基醚(Methy1 tertiary butyl ether,MTBE)对大鼠大脑皮质Fos蛋白、三磷酸腺苷(ATP)酶、神经元及神经胶质细胞形态结构的影响,探讨甲基叔丁基醚对中枢神经系统的急性毒作用机制。方法66只健康成年SD大鼠,腹腔注射原液染毒,染毒剂量为0,700,1 050,1 400mg/(kg.bw)。用免疫组织化学、分光光度法、尼氏(Nissl)染色法观察染毒后0.5,1.0,2.0,4.0,8.0 h时MTBE对大鼠大脑皮质的影响。结果对照组大脑皮质神经元内仅有少量Fos蛋白表达,实验组Fos蛋白表达明显增多,其相对灰度值随着MTBE染毒剂量的增加而增多;Fos蛋白在染毒后0.5 h时表达开始增加,1.0 h达到高峰,2.0 h时开始减弱,8.0 h时恢复至对照组水平。MTBE染毒后1.0 h大脑皮质ATP酶的活性明显降低(P<0.05),染毒后8.0h其活性无明显变化(P>0.05)。实验组大脑神经元、神经胶质细胞光镜下形态结构未见明显改变。结论MTBE可毒作用于大脑;MTBE可降低大鼠大脑ATP酶的活性,改变神经细胞能量代谢;MTBE急性染毒时,大脑神经元、神经胶质细胞光镜下其形态结构未见明显改变。
Objective To explore the acute toxic mechanism of methyl tertiary butyl ether（MTBE） by detecting the Fos protein expression, the activity of Na^＋ , K^＋ - ATPase, Ca^2＋ - ATPase and the structure changes of nerve cell and neuroglia in the cerebral cortex Methods 66 adult SD rats were randomly divided into control and experimental groups. The MTBE exposure dose was 0,700, 1 050, 1 400 mg/（kg·bw） and the control group with the same doses of normal saline njeeted into abdominal cavity. The immunohistochemical method （SABC method） was used to detect the Fos protein in the cerebral cortex. The spectrophotometric method was used to measure the activity change of the Na^＋ , K^＋ - ATPase, Ca^2＋ - ATPase in the cerebral cortex. The pathological study （Nissl staining） and immunohistochemieal method （GFAP） were performed with optical microscope to observe histopathological changes in nerve cell and neuroglia cell of the cerebral cortex. Results The expression of the Fos protein in cerebral cortex was increased when exposed to MTBE 700, 1 050, 1400 mg/（kg·bw）. The expression of the Fos protein in cerebral cortex was changed in different time（0.5, 1.0, 2.0, 4.0, 8.0 h） after exposed to MTBE [1 050 mg（kg· bw）） The expression of the Fos began to increase in 0.5 h and reached the peak at 1.0 h, and then reduced in 2.0h, recovred to normal level in 8.0 h. The activity of Na^＋, K^＋ - ATPase, Ca^2＋ ATPase in cerebral cortex decreased dramatically in 1.0 h compared with the control （ P 〈 0.05）. The activity of Na^＋, K^＋ - ATPase, Ca^2＋ - ATPase recovered to normal level （P 〉 0.05） in 8.0 h. The structure change was not observed obviously in the nerve cell and the neuroglia. Conclusion The acute toxic effect of MTBE can be resulted in the cerebrum. MTBE can reduce the activity of the Na^＋, K^＋ - ATPase, Ca^2＋ - ATPase, affect the energy metabolism of the nerve cell following the inhibitory action of cerebrum. The structure changes of the nerve cell and the neuroglia are not observed obviously when MTBE has an acute toxic effect on cerebrum.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2008年第7期840-842,共3页
Chinese Journal of Public Health
基金
湖南省卫生厅科研基金(B2005090)
