摘要
目的:探讨熟地黄水提物含药血清对人脐静脉内皮细胞-1(HUVECs-1)增殖的影响及其机制。方法:熟地黄水提物3,6,10 g·kg^(-1)分别给SD大鼠灌胃1周,取血制备含药血清,加入血管内皮细胞培养液。采用MTT法观察血清对HUVECs-1增殖的影响,免疫细胞化学染色和Western blot检测血清对HUVECs-1表达红细胞生成素(EPO)的影响。结果:与相同浓度的NS血清组比较,3,6 g·kg^(-1)地黄水提液含药血清组HUVEC-1吸光度显著升高(P<0.05)。免疫组化结果显示,3,6 g·kg^(-1)熟地黄水提物5%含药血清组与相同浓度NS血清组比较,3,6,10g·kg^(-1)熟地黄水提物10%,20%含药血清组与相同浓度NS血清组比较,EPO免疫反应阳性细胞平均吸光度均显著增加(P<0.05)。Western blot分析显示:与NS血清组比较,6 g·kg^(-1)熟地黄水提物相同浓度含药血清各组EPO相对条带吸光度值(IOD)均显著增高(P<0.05)。结论:中药熟地黄水提物含药血清可上调血管内皮细胞EPO表达,对血管内皮细胞有显著的促增殖作用。
Objective： To explore the efficacy of the rehmannia root decoction containing serum in enhencing the proliferation of HUVECs-1 and EPO expression and to give libratory evidence for the tonifying blood and kidney therapy with rehmannla root. Method： Twenty-four male Sprague-Dawley rats were randomly devided into 4 group and administrered by gastrogavage rehmannia root decoction of 3, 6, 10 g· kg^-1 once a day for 1 weeks. Using serum pharmacologic method, the proliferation of HUVECs-1 cell was determined by MTT ehromatometry at 48 h points by co-culturing with medicated serum containing different concentration of rehmannia root decoction. The expresstion of EPO on HUVECs-1 were observed by immunity cytochemistry and Western blot. NS serum was taken as control. Result： After the HUVECs-1 cultivating with medicated serum for 48 h, compared with NS serum, 20% serum containing reh- mannia root （6 g · kg^-1 ） could obviously increase proliferation of HUVEC-1 （P 〈0. 05） and increase the level of the expresstion of EPO on HUVECs-1 （ P 〈 0. 05 ）. Conclusion ： Rehmannia root extract has good actions of proliferation of HUVECs-1 and increase EPO expression, which is the mechanisms of nourishing yin and blood and tonifying essence of rehmannia root.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第13期1579-1582,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(30070915)
西南大学博士基金项目(104290-20710906)
