摘要
采用葡萄糖还原氯金酸的方法制得粒径约15nm的纳米金,在pH3.54酸性介质中它可以与盐酸普鲁卡因作用形成体积更大的纳米金聚集体。这种聚集体的形成可以使纳米金的瑞利共振散射强度急剧增强,其最大散射峰位于354nm左右。在适当的条件下,相对散射强度(ΔI)与盐酸普鲁卡因的浓度成正比。采用标准加入法,对针剂中的普鲁卡因进行测定,得到满意结果。线性范围0～40μg/L(r=0.9996);对含盐酸普鲁卡因30μg/L的溶液平行测定的相对标准偏差为2.51%(n=11);检出限(3σ)为16ng/L;测定限(10σ)为54ng/L。
Nano-gold colloid was prepared by chemical reduction of HAuCl4 by glucose（C6H12O6） and the gold particles of 15nm were obtained.The nanogold particles became super-molecular when combined with procaine hydrochloride at pH 3.54,showing a resonance Rayleigh scattering peak at 354 nm.In optimum conditions,there was a linear relationship between scattering intensity ΔI and the concentration of procaine hydrochloride.A standard adding method was used for the determination of procaine hydrochloride injection.A detection limits（3σ）of 16 ng/L was obtained in the linear range of 0-40 μg/L（r=0.9996） with the RSD of 2.5%（30 μg/L,n=11） and the lowest quantification concentration of 54 ng/L.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2008年第7期995-998,共4页
Chinese Journal of Analytical Chemistry
基金
国家自然科学基金资助项目(No20675011)
关键词
纳米金
盐酸普鲁卡因
共振瑞利散射
Nanogold,procaine hydrochloride,resonance Rayleigh scattering
