摘要
目的建立一种对苯安莎类抗生素特异的颜色反应早期鉴别方法。方法根据碱性处理苯安莎类抗生素,使其安莎链与苯环连接的酰胺键开裂后化合物呈紫色的原理,建立了将发酵液粗提品进行薄层色谱层析后采用2.0mol/L NaOH溶液喷涂显色的早期鉴别方法;以格尔德霉素为例,在硅胶板上的检测灵敏度为4μg。结论采用该颜色鉴别反应方法:1对两株确证为新的格尔德霉素产生菌Streptomyces sp.4-4以及Streptomyces sp.3-57进行了佐证;2对吸水链霉菌Streptomyces hygroscopicus 17997的一株格尔德霉素生物合成后修饰基因阻断变株CT-1中产生的格尔德霉素前体物及其类似物,在硅胶板上进行了快速定位和初步鉴别。
Objective Establishing a simple and sensitive method for early preliminary discrimination of benzenic ansamycins and their derivatives. Method Most natural benzenic ansamycins are yellow compounds, some are white or colorless compounds. After treatment of alkali such as NaOH or sodium methoxide, which breaks the amide bond formed between the benzenic amino-group and the ansa-bridgers terminal carboxygroup, benzenic ansamycins such as geldanamycin changed its color from yellow to purple. The minimal detection limit of this method on the silica gel plate for geldanamycin was 4μg with NaOH （2.0mol/L） sprayed. Results This color change reaction method was used successfully for early preliminary discrimination of geldanamycin produced by two soil-isolated novel strains, Streptomyces sp. 4-4 and Streptomyces sp. 3-57, with ansamycin producing potential（s）, and fast location and preliminary discrimination on silica gel plate of geldanamycin derivatives produced by CT-1, a mutant strain of Streptomyces hygroscopicus 17997 with one of the geldanamycin biosynthetic tailoring genes being disrupted.
出处
《中国抗生素杂志》
CAS
CSCD
北大核心
2008年第7期403-406,共4页
Chinese Journal of Antibiotics
基金
国家自然科学基金项目(30570039)
关键词
苯安莎类抗生素
酰胺键
颜色反应
鉴别
Benzenic ansamycins
Amide bond
Color reaction
Discrimination
